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题名: Application of matrix solid-phase dispersion methodology to the extraction of endogenous peptides from porcine hypothalamus samples for MS and LC-MS analysis
作者: Cai, Xiaoming1;  Wang, Chaoran1;  Xu, Junyan1;  Xue, Xingya1;  Zhang, Xiuli1;  Liang, Xinmiao1
关键词: Endogenous peptides ;  Matrix solid-phase dispersion extraction ;  NanoLC-MS/MS ;  Peptide extraction ;  Porcine hypothalamus
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2011-03-15
DOI: 10.1016/j.jchromb.2011.01.038
卷: 879, 期:9-10, 页:657-661
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: In this study, we investigated a novel application of matrix solid-phase dispersion (MSPD) methodology for the extraction of endogenous peptides from porcine hypothalamus tissue samples. Several experimental factors of the MSPD procedure were examined. Finally, silica-based octadecyl was chosen as dispersing material and blended with 0.25 g porcine hypothalamus at a ratio of 5, and 10 mL of 60% acetonitrile with 0.2% formic acid in water was chosen as the extraction and elution solvent. This MSPD extraction method was compared to the classic acid extraction method. More peaks were observed in the MSPD extracts (74 +/- 5) by MALDI-TOF MS than in acid extracts (34 +/- 5). Moreover, 14 potential endogenous peptides were identified in the MSPD extracts after nanoLC-MS/MS analysis, while only 2 endogenous peptides in the acid extracts. These results indicated that MSPD could be employed as a simple and efficient method for the extraction of endogenous peptides from tissues. (C) 2011 Elsevier B.V. All rights reserved.
关键词[WOS]: TANDEM MASS-SPECTROMETRY ;  LIQUID-CHROMATOGRAPHY ;  NEUROPEPTIDOMICS ;  OPTIMIZATION ;  PEPTIDOMICS ;  DISCOVERY ;  PLASMA ;  MS/MS ;  EGGS
语种: 英语
WOS记录号: WOS:000288723500016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142336
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China

Recommended Citation:
Cai, Xiaoming,Wang, Chaoran,Xu, Junyan,et al. Application of matrix solid-phase dispersion methodology to the extraction of endogenous peptides from porcine hypothalamus samples for MS and LC-MS analysis[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(9-10):657-661.
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