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Study on membrane characteristics of alginate-chitosan microcapsule with cell growth
Yu, Weiting2; Song, Huiyi2; Zheng, Guoshuang2; Liu, Xiudong1; Zhang, Ying2; Ma, Xiaojun2
KeywordAlginate-chitosan Microcapsule Membrane Characteristics Microencapsulated Cell Culture Apparent Membrane
Source PublicationJOURNAL OF MEMBRANE SCIENCE
2011-07-15
DOI10.1016/j.memsci.2011.04.053
Volume377Issue:1-2Pages:214-220
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Technology ; Physical Sciences
WOS SubjectEngineering, Chemical ; Polymer Science
WOS Research AreaEngineering ; Polymer Science
WOS KeywordOLIGOCHITOSAN MICROCAPSULES ; INTESTINAL DELIVERY ; SWELLING BEHAVIOR ; PREPARED CAPSULE ; MOLECULAR-WEIGHT ; IN-VITRO ; RELEASE ; MICROPARTICLES ; ENCAPSULATION ; MICROSPHERES
AbstractAlginate-chitosan (AC) microcapsule with polyelectrolyte complex (PEC) membrane has been attractive in biotechnological area such as cell immobilization fermentation. The PEC membrane has been known important to control microcapsule performance, but the knowledge was mainly obtained with empty or drug-loaded microcapsules. In this paper, the emphasis was to explore the influence of cell growth on PEC membrane characteristics, that is, to study the membrane characteristics in dynamic process. AC microcapsule entrapping yeast cells (Saccharomyces cerevisiae BY4741) was used as model, and the PEC membrane characteristics including membrane thickness, swelling behavior, permeability, and component were studied during cell culture process. It was found that both the membrane thickness and volume swelling degree of AC microcapsule increased, however, membrane permeability decreased with the growth of entrapped yeast cells. Fluorescence-labeling studies suggested the concentrated alginate layer squeezed by the proliferated cells and macromolecular metabolites layer secreted by proliferated cells contributed to above increment. The former PEC membrane, together with the new layers, constituted the 'apparent membrane', which increased the diffusion resistance of substances. (C) 2011 Elsevier B.V. All rights reserved.
Language英语
WOS IDWOS:000292580000022
Citation statistics
Cited Times:34[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/142364
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Univ, Coll Environm & Chem Engn, Dalian 116622, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Yu, Weiting,Song, Huiyi,Zheng, Guoshuang,et al. Study on membrane characteristics of alginate-chitosan microcapsule with cell growth[J]. JOURNAL OF MEMBRANE SCIENCE,2011,377(1-2):214-220.
APA Yu, Weiting,Song, Huiyi,Zheng, Guoshuang,Liu, Xiudong,Zhang, Ying,&Ma, Xiaojun.(2011).Study on membrane characteristics of alginate-chitosan microcapsule with cell growth.JOURNAL OF MEMBRANE SCIENCE,377(1-2),214-220.
MLA Yu, Weiting,et al."Study on membrane characteristics of alginate-chitosan microcapsule with cell growth".JOURNAL OF MEMBRANE SCIENCE 377.1-2(2011):214-220.
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