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题名: Sequential microfluidic droplet processing for rapid DNA extraction
作者: Pan, Xiaoyan1, 2;  Zeng, Shaojiang1, 2;  Zhang, Qingquan1, 2;  Lin, Bingcheng1;  Qin, Jianhua1
关键词: DNA extraction ;  Droplet processing ;  Droplet splitting ;  Reagent addition
刊名: ELECTROPHORESIS
发表日期: 2011-11-01
DOI: 10.1002/elps.201100078
卷: 32, 期:23, 页:3399-3405
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: This work describes a novel droplet-based microfluidic device, which enables sequential droplet processing for rapid DNA extraction. The microdevice consists of a droplet generation unit, two reagent addition units and three droplet splitting units. The loading/washing/elution steps required for DNA extraction were carried out by sequential microfluidic droplet processing. The movement of superparamagnetic beads, which were used as extraction supports, was controlled with magnetic field. The microdevice could generate about 100 droplets per min, and it took about 1?min for each droplet to perform the whole extraction process. The extraction efficiency was measured to be 46% for ?-DNA, and the extracted DNA could be used in subsequent genetic analysis such as PCR, demonstrating the potential of the device for fast DNA extraction.
关键词[WOS]: DEVICES ;  PLUGS ;  ENCAPSULATION ;  SYSTEM ;  SIZE
语种: 英语
WOS记录号: WOS:000298300900013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142365
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

Recommended Citation:
Pan, Xiaoyan,Zeng, Shaojiang,Zhang, Qingquan,et al. Sequential microfluidic droplet processing for rapid DNA extraction[J]. ELECTROPHORESIS,2011,32(23):3399-3405.
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