DICP OpenIR
Sequential microfluidic droplet processing for rapid DNA extraction
Pan, Xiaoyan1,2; Zeng, Shaojiang1,2; Zhang, Qingquan1,2; Lin, Bingcheng1; Qin, Jianhua1
KeywordDna Extraction Droplet Processing Droplet Splitting Reagent Addition
Source PublicationELECTROPHORESIS
2011-11-01
DOI10.1002/elps.201100078
Volume32Issue:23Pages:3399-3405
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordDEVICES ; PLUGS ; ENCAPSULATION ; SYSTEM ; SIZE
AbstractThis work describes a novel droplet-based microfluidic device, which enables sequential droplet processing for rapid DNA extraction. The microdevice consists of a droplet generation unit, two reagent addition units and three droplet splitting units. The loading/washing/elution steps required for DNA extraction were carried out by sequential microfluidic droplet processing. The movement of superparamagnetic beads, which were used as extraction supports, was controlled with magnetic field. The microdevice could generate about 100 droplets per min, and it took about 1?min for each droplet to perform the whole extraction process. The extraction efficiency was measured to be 46% for ?-DNA, and the extracted DNA could be used in subsequent genetic analysis such as PCR, demonstrating the potential of the device for fast DNA extraction.
Language英语
WOS IDWOS:000298300900013
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/142365
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Pan, Xiaoyan,Zeng, Shaojiang,Zhang, Qingquan,et al. Sequential microfluidic droplet processing for rapid DNA extraction[J]. ELECTROPHORESIS,2011,32(23):3399-3405.
APA Pan, Xiaoyan,Zeng, Shaojiang,Zhang, Qingquan,Lin, Bingcheng,&Qin, Jianhua.(2011).Sequential microfluidic droplet processing for rapid DNA extraction.ELECTROPHORESIS,32(23),3399-3405.
MLA Pan, Xiaoyan,et al."Sequential microfluidic droplet processing for rapid DNA extraction".ELECTROPHORESIS 32.23(2011):3399-3405.
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