DICP OpenIR
Sequential microfluidic droplet processing for rapid DNA extraction
Pan, Xiaoyan1,2; Zeng, Shaojiang1,2; Zhang, Qingquan1,2; Lin, Bingcheng1; Qin, Jianhua1
关键词Dna Extraction Droplet Processing Droplet Splitting Reagent Addition
刊名ELECTROPHORESIS
2011-11-01
DOI10.1002/elps.201100078
32期:23页:3399-3405
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]DEVICES ; PLUGS ; ENCAPSULATION ; SYSTEM ; SIZE
英文摘要This work describes a novel droplet-based microfluidic device, which enables sequential droplet processing for rapid DNA extraction. The microdevice consists of a droplet generation unit, two reagent addition units and three droplet splitting units. The loading/washing/elution steps required for DNA extraction were carried out by sequential microfluidic droplet processing. The movement of superparamagnetic beads, which were used as extraction supports, was controlled with magnetic field. The microdevice could generate about 100 droplets per min, and it took about 1?min for each droplet to perform the whole extraction process. The extraction efficiency was measured to be 46% for ?-DNA, and the extracted DNA could be used in subsequent genetic analysis such as PCR, demonstrating the potential of the device for fast DNA extraction.
语种英语
WOS记录号WOS:000298300900013
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/142365
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Pan, Xiaoyan,Zeng, Shaojiang,Zhang, Qingquan,et al. Sequential microfluidic droplet processing for rapid DNA extraction[J]. ELECTROPHORESIS,2011,32(23):3399-3405.
APA Pan, Xiaoyan,Zeng, Shaojiang,Zhang, Qingquan,Lin, Bingcheng,&Qin, Jianhua.(2011).Sequential microfluidic droplet processing for rapid DNA extraction.ELECTROPHORESIS,32(23),3399-3405.
MLA Pan, Xiaoyan,et al."Sequential microfluidic droplet processing for rapid DNA extraction".ELECTROPHORESIS 32.23(2011):3399-3405.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Pan, Xiaoyan]的文章
[Zeng, Shaojiang]的文章
[Zhang, Qingquan]的文章
百度学术
百度学术中相似的文章
[Pan, Xiaoyan]的文章
[Zeng, Shaojiang]的文章
[Zhang, Qingquan]的文章
必应学术
必应学术中相似的文章
[Pan, Xiaoyan]的文章
[Zeng, Shaojiang]的文章
[Zhang, Qingquan]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。