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题名: The proteome analysis of oleaginous yeast Lipomyces starkeyi
作者: Liu, Hongwei1, 2;  Zhao, Xin2, 3;  Wang, Fangjun1, 2;  Jiang, Xinning1, 2;  Zhang, Sufang3;  Ye, Mingliang1, 3;  Zhao, Zongbao K.3;  Zou, Hanfa1, 3
关键词: oleaginous yeast ;  Lipomyces starkeyi ;  microbial lipid ;  nitrogen deficiency ;  proteomics
刊名: FEMS YEAST RESEARCH
发表日期: 2011-02-01
DOI: 10.1111/j.1567-1364.2010.00687.x
卷: 11, 期:1, 页:42-51
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology ;  Microbiology ;  Mycology
研究领域[WOS]: Biotechnology & Applied Microbiology ;  Microbiology ;  Mycology
英文摘要: Oleaginous yeast Lipomyces starkeyi, a species in the Saccharomycetales order, has the capability to accumulate over 70% of its cell biomass as lipid under defined culture conditions. In this study, analysis of L. starkeyi AS 2.1560 proteome samples from different culture stages during a typical lipid production process was performed using an online multidimensional mu RPLC/MS/MS method. Data searching against the proteome database of the yeast Saccharomyces cerevisiae led to the identification of 289 protein hits. Further comparative and semi-quantitative analysis under more stringent criteria revealed 81 proteins with significant expression-level changes. Among them, 52 proteins were upregulated and 29 proteins were downregulated. Gene ontology annotation indicated that global responses occurred when cells were exposed to the nitrogen deficiency environment for lipid production. Protein hits were annotated and largely concerned metabolic processes for alternative nitrogen sources usage or lipid accumulation. Many of the downregulated proteins were related to glycolysis, whereas the majority of the upregulated proteins were involved in proteolysis and peptidolysis, carbohydrate metabolism and lipid metabolism. Insights were provided in terms of cellular responses to nutrient availability as well as the basic biochemistry of lipid accumulation. This work presented potentially valuable information for understanding the biochemical events related to microbial oleaginity and rational engineering of oleaginous yeasts.
关键词[WOS]: FED-BATCH CULTURE ;  BIODIESEL PRODUCTION ;  LIPID-ACCUMULATION ;  RHODOSPORIDIUM-TORULOIDES ;  SACCHAROMYCES-CEREVISIAE ;  YARROWIA-LIPOLYTICA ;  MICROORGANISMS ;  GLUCOSE ;  PURIFICATION ;  BIOLOGY
语种: 英语
WOS记录号: WOS:000286212800005
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142388
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.CAS, Dalian Inst Chem Phys, CAS Key Lab Separat Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.CAS, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China

Recommended Citation:
Liu, Hongwei,Zhao, Xin,Wang, Fangjun,et al. The proteome analysis of oleaginous yeast Lipomyces starkeyi[J]. FEMS YEAST RESEARCH,2011,11(1):42-51.
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