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题名: Analysis of Phospholipids in Crucian Carp(Carassius auratus) Muscle by Offline HPLC-MALDI-TOF MS
作者: Li Guo-chen1;  Wang Yan-hong1;  Wu Ren-an2;  Wang Shi-cheng1
关键词: Phospholipid ;  Phosphatidylcholine ;  Phosphatidylethanolamine ;  Sphingomyelin ;  HPLC ;  MALDI ;  Crucian Carp
刊名: CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
发表日期: 2011-05-01
卷: 27, 期:3, 页:371-374
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: The interest in the analysis of phospholipids(PLs), especially phosphatidylcholine(PC), has been increasing due to the importance of them in biochemistry as well as in industry. A method was reported based on the offline combination of MALDI-TOF MS and normal-phase HPLC for analyzing PLs extracted from crucian carp. Total PLs of crucian carp were extracted and then separated by HPLC before the collected subfractions were analyzed by MALDI-TOF MS. The mass spectra obtained show peaks of H(+), Na(+) and K(+) adducts of PC molecules. It is shown that the prior separation of PLs by HPLC is highly necessary to remove the signal suppressing and to avoid the possible overlapping. With this method, 9 possible PC molecules in crucian carp and the corresponding fatty acid compositions were given from the well-resolved mass spectra.
关键词[WOS]: FLIGHT MASS-SPECTROMETRY ;  ASSISTED LASER-DESORPTION ;  LIQUID-CHROMATOGRAPHY ;  EGG-YOLK ;  MATRIX ;  IONIZATION ;  PEPTIDES ;  LIPIDS ;  LIPIDOMICS ;  MIXTURES
语种: 英语
WOS记录号: WOS:000291505100007
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142389
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Inspect & Testing Ctr Agroprod Safety & Environm, Inst Appl Ecol, Shenyang 110016, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Li Guo-chen,Wang Yan-hong,Wu Ren-an,et al. Analysis of Phospholipids in Crucian Carp(Carassius auratus) Muscle by Offline HPLC-MALDI-TOF MS[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2011,27(3):371-374.
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