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Analysis of Phospholipids in Crucian Carp(Carassius auratus) Muscle by Offline HPLC-MALDI-TOF MS
Li Guo-chen1; Wang Yan-hong1; Wu Ren-an2; Wang Shi-cheng1
关键词Phospholipid Phosphatidylcholine Phosphatidylethanolamine Sphingomyelin Hplc Maldi Crucian Carp
刊名CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
2011-05-01
27期:3页:371-374
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]FLIGHT MASS-SPECTROMETRY ; ASSISTED LASER-DESORPTION ; LIQUID-CHROMATOGRAPHY ; EGG-YOLK ; MATRIX ; IONIZATION ; PEPTIDES ; LIPIDS ; LIPIDOMICS ; MIXTURES
英文摘要The interest in the analysis of phospholipids(PLs), especially phosphatidylcholine(PC), has been increasing due to the importance of them in biochemistry as well as in industry. A method was reported based on the offline combination of MALDI-TOF MS and normal-phase HPLC for analyzing PLs extracted from crucian carp. Total PLs of crucian carp were extracted and then separated by HPLC before the collected subfractions were analyzed by MALDI-TOF MS. The mass spectra obtained show peaks of H(+), Na(+) and K(+) adducts of PC molecules. It is shown that the prior separation of PLs by HPLC is highly necessary to remove the signal suppressing and to avoid the possible overlapping. With this method, 9 possible PC molecules in crucian carp and the corresponding fatty acid compositions were given from the well-resolved mass spectra.
语种英语
WOS记录号WOS:000291505100007
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被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/142389
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Inspect & Testing Ctr Agroprod Safety & Environm, Inst Appl Ecol, Shenyang 110016, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
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GB/T 7714
Li Guo-chen,Wang Yan-hong,Wu Ren-an,et al. Analysis of Phospholipids in Crucian Carp(Carassius auratus) Muscle by Offline HPLC-MALDI-TOF MS[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2011,27(3):371-374.
APA Li Guo-chen,Wang Yan-hong,Wu Ren-an,&Wang Shi-cheng.(2011).Analysis of Phospholipids in Crucian Carp(Carassius auratus) Muscle by Offline HPLC-MALDI-TOF MS.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,27(3),371-374.
MLA Li Guo-chen,et al."Analysis of Phospholipids in Crucian Carp(Carassius auratus) Muscle by Offline HPLC-MALDI-TOF MS".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 27.3(2011):371-374.
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