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题名: Phylogenetic diversity and characterization of 2-haloacid degrading bacteria from the marine sponge Hymeniacidon perlevis
作者: Huang, Jianyu1, 2;  Xin, Yanjuan1;  Cao, Xupeng1;  Zhang, Wei1, 3, 4
关键词: Bacteria ;  Marine sponge ;  2-haloacid dehalogenase ;  Salt tolerance ;  Enantioselectivity
刊名: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
发表日期: 2011-08-01
DOI: 10.1007/s11274-010-0636-8
卷: 27, 期:8, 页:1787-1794
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: A total of 139 2-haloacid degrading bacteria strains were isolated from the marine sponge Hymeniacidon perlevis using a modified enrichment medium and a pH indicator method. After screening on indicator agar and 2-chloropropionic acid (2-CPA) liquid medium, 11 isolates with high degrading activities were characterized and initially identified. Seven of the 11 isolates were able to degrade 2-CPA at 8% salt, and four isolates (DEH 66, DEH 99, DEH125 and DEH138) degraded 2-CPA at 15% salt. Eight of the 11 isolates utilized all four types of organohalogen compounds used in this study. The DEH99 and DEH138 isolates exhibited the best enantioselectivity towards (S)-2-chloropropionic acid (S-CPA) and (R)-2-chloropropionic acid (R-CPA), respectively. The dehalogenase activities of DEH84 against racemic CPA, DEH99 against S-CPA, DEH138 against R-CPA and DEH130 against racemic CPA were 0.16U/mg, 0.06U/mg, 0.12U/mg and 0.19U/mg, respectively. Based on 16S rRNA sequence analysis, the 11 isolates were clustered into the Rhodobacteraceae family of alpha-proteobacteria and the Pseudomonadaceae family of gamma-proteobacteria. To our knowledge, this is the first report detailing the isolation of organisms of Pseudomonas stuzeri sp. and the Rhodobacteraceae family with 2-haloacid dehalogenase activity from marine sponges.
关键词[WOS]: DL-2-HALOACID DEHALOGENASE ;  MICROBIAL-DEGRADATION ;  HALOACID DEHALOGENASE ;  PSEUDOMONAS SP ;  GENE ;  PURIFICATION ;  MICROORGANISMS ;  BIOCHEMISTRY ;  SEQUENCE ;  PROTEIN
语种: 英语
WOS记录号: WOS:000292984000006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142390
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Dalian Univ Technol, Minist Educ, Key Lab Ind Ecol & Environm Engn, Sch Environm Sci & Technol, Dalian 116024, Peoples R China
3.Flinders Univ S Australia, Sch Med, Adelaide, SA 5042, Australia
4.Flinders Univ S Australia, Flinders Ctr Marine Bioproc & Bioprod FCMB2, Adelaide, SA 5042, Australia

Recommended Citation:
Huang, Jianyu,Xin, Yanjuan,Cao, Xupeng,et al. Phylogenetic diversity and characterization of 2-haloacid degrading bacteria from the marine sponge Hymeniacidon perlevis[J]. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY,2011,27(8):1787-1794.
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