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Phylogenetic diversity of Gram-positive bacteria cultured from Antarctic deep-sea sponges
Xin, Yanjuan1,2; Kanagasabhapathy, Manmadhan1; Janussen, Dorte5,6; Xue, Song1; Zhang, Wei1,3,4
KeywordGram-positive Bacteria Pks Antarctic Porifera Siliceous Sponges 16s Rrna
Source PublicationPOLAR BIOLOGY
2011-10-01
DOI10.1007/s00300-011-1009-y
Volume34Issue:10Pages:1501-1512
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiodiversity Conservation ; Ecology
WOS Research AreaBiodiversity & Conservation ; Environmental Sciences & Ecology
WOS KeywordBARRIER-REEF SPONGE ; ABYSSAL WEDDELL SEA ; SOIL BACTERIA ; PSEUDOCERATINA-CLAVATA ; SELECTIVE ISOLATION ; MARINE-SEDIMENTS ; GENE-SEQUENCES ; SOUTHERN-OCEAN ; ACTINOBACTERIA ; CULTIVATION
AbstractGram-positive bacteria, specifically actinobacteria and members of the order Bacillales, are well-known producers of important secondary metabolites. Little is known about the diversity of Gram-positive bacteria associated with Antarctic deep-sea sponges. In this study, cultivation-based approaches were applied to investigate the Gram-positive bacteria associated with the Antarctic sponges Rossella nuda, Rossella racovitzae (Porifera: Hexactinellida), and Myxilla mollis, Homaxinella balfourensis, Radiella antarctica (Porifera: Demospongiae). In total, 46 Gram-positive strains were cultured. Phylogenetic analysis revealed that 24 strains were affiliated with the Actinobacteria, including six genera Streptomyces, Nocardiopsis, Pseudonocardia, Dietzia, Brachybacterium, and Brevibacterium. The other 22 strains were affiliated with the Firmicutes, and among them two (V17-1 and V179-1) only shared 92-95% 16S rRNA gene sequence identity with the nearest type strain. To our knowledge, this is the first report on the isolation of strains belonging to genera Dietzia and Brevibacterium from Antarctic sponges. All of the 46 strains were PCR screened for genes encoding polyketide synthases (PKS), and a selection of 36 isolates were used in subsequent bioassay analyses. Eighty-eight percentage of the isolates that possess a PKS gene were active against at least one test organism. The study confirms the existence of diverse bacteria in Antarctic sponges and their potential for producing active compounds.
Language英语
WOS IDWOS:000297202300008
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/142428
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Flinders Univ S Australia, Sch Med, Flinders Ctr Marine Bioproc & Bioprod, Bedford Pk, SA 5042, Australia
4.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Bedford Pk, SA 5042, Australia
5.Senckenberg Res Inst, Sect Marine Invertebrates 1, D-60325 Frankfurt, Germany
6.Natl Museum, D-60325 Frankfurt, Germany
Recommended Citation
GB/T 7714
Xin, Yanjuan,Kanagasabhapathy, Manmadhan,Janussen, Dorte,et al. Phylogenetic diversity of Gram-positive bacteria cultured from Antarctic deep-sea sponges[J]. POLAR BIOLOGY,2011,34(10):1501-1512.
APA Xin, Yanjuan,Kanagasabhapathy, Manmadhan,Janussen, Dorte,Xue, Song,&Zhang, Wei.(2011).Phylogenetic diversity of Gram-positive bacteria cultured from Antarctic deep-sea sponges.POLAR BIOLOGY,34(10),1501-1512.
MLA Xin, Yanjuan,et al."Phylogenetic diversity of Gram-positive bacteria cultured from Antarctic deep-sea sponges".POLAR BIOLOGY 34.10(2011):1501-1512.
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