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题名: Isolation, characterization and identification of a Paracoccus sp 2-haloacid-degrading bacterium from the marine sponge Hymeniacidon perlevis
作者: Huang, Jianyu1, 2;  Xin, Yanjuan1, 2;  Zhang, Wei1, 3, 4
关键词: 2-haloacid dehalogenase ;  marine sponge ;  stereoselectivity ;  Hymeniacidon perlevis
刊名: JOURNAL OF BASIC MICROBIOLOGY
发表日期: 2011-06-01
DOI: 10.1002/jobm.201000205
卷: 51, 期:3, 页:318-324
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
英文摘要: A 2-haloacid dehalogenase-producing bacterium, designated DEH99, was isolated from the marine sponge Hymeniacidon perlevis using a modified enrichment medium and a pH indicator method. DEH99 could degrade only half of the racemic mixture 2-chloropropionic acid (2-CPA) in the medium. The dehalogenase of DEH99 was further determined to be a (S)-2-haloacid dehalogenase, which can degrade 2-CPA, 2-bromopropionic acid (2-BPA), and iodoacetic acid. The gene encoding the (S)-2-haloacid dehalogenase was partially sequenced and classified into the Group II family. The 2-haloacid dehalogenase showed the highest sequence similarity (77% with 21% query coverage) to the haloacid dehalogenase (dhlB) gene of Xanthobacter autotrophicus. A phylogenetic analysis of the 16S rDNA sequence demonstrated that the isolate DEH99 is a member of the genus Paracoccus. To our knowledge, this is the first report detailing the isolation of a strain of genus Paracoccus having 2-haloacid dehalogenase activity from marine sponges.
关键词[WOS]: ACID DEHALOGENASE ;  HALOACID DEHALOGENASE ;  PSEUDOMONAS-PUTIDA ;  GENE ;  PURIFICATION ;  SEQUENCE ;  CLONING
语种: 英语
WOS记录号: WOS:000291107500011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142430
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Flinders Univ S Australia, Flinders Ctr Marine Bioproc & Bioprod, Sch Med, Adelaide, SA, Australia
4.Flinders Univ S Australia, Dept Med Biotechnol, Sch Med, Adelaide, SA, Australia

Recommended Citation:
Huang, Jianyu,Xin, Yanjuan,Zhang, Wei. Isolation, characterization and identification of a Paracoccus sp 2-haloacid-degrading bacterium from the marine sponge Hymeniacidon perlevis[J]. JOURNAL OF BASIC MICROBIOLOGY,2011,51(3):318-324.
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