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题名: Liquid chromatography/mass spectrometry-based metabolic profiling to elucidate chemical differences of tobacco leaves between Zimbabwe and China
作者: Li, Qinghua1, 2;  Zhao, Chunxia1;  Li, Yong1;  Chang, Yuwei1;  Wu, Zeming1;  Pang, Tao3;  Lu, Xin1;  Wu, Yi3;  Xu, Guowang1
关键词: Chemical components ;  Liquid chromatography ;  Mass spectrometry ;  Metabolic profiling ;  Tobacco leaves
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2011
DOI: 10.1002/jssc.201000652
卷: 34, 期:2, 页:119-126
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: DAn approach was developed for extracting and analyzing the chemical components of tobacco leaves based on solvent extraction and rapid & resolution liquid chromatography/quadrupole time-of-flight mass spectrometry analysis. Two solvents with different polarities were used to extract hydrophilic components and hydrophobic components, respectively, the combined analytical data can provide a "global'' view of metabolites. Based on the evaluation of parallel samples, it was found that this approach provided good repeatability, accurate and reliable profiling data, and is suitable for the metabolomics study of tobacco leaves. In order to find the chemical component differences of tobacco leaves, 56 samples from Zimbabwe and China were analyzed using the developed method. The metabolite data were processed by multivariate statistic technique; an obvious group classification between Zimbabwe and China was observed, 14 significantly changed compounds were found, and 9 of them were identified.
关键词[WOS]: MASS-SPECTROMETRY ;  GAS-CHROMATOGRAPHY ;  PLANT METABOLOMICS ;  CROSS-VALIDATION ;  IDENTIFICATION ;  EXTRACTION ;  STRATEGY ;  BIOLOGY ;  MODELS ;  SMOKE
语种: 英语
WOS记录号: WOS:000287603400001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142477
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Hongyun Tobacco Grp Co Ltd, Kunming Cigarette Factory, Kunming, Peoples R China
3.Yunnan Acad Tobacco Agr Sci, Yuxi, Peoples R China

Recommended Citation:
Li, Qinghua,Zhao, Chunxia,Li, Yong,et al. Liquid chromatography/mass spectrometry-based metabolic profiling to elucidate chemical differences of tobacco leaves between Zimbabwe and China[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(2):119-126.
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