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Liquid chromatography/mass spectrometry-based metabolic profiling to elucidate chemical differences of tobacco leaves between Zimbabwe and China
Li, Qinghua1,2; Zhao, Chunxia1; Li, Yong1; Chang, Yuwei1; Wu, Zeming1; Pang, Tao3; Lu, Xin1; Wu, Yi3; Xu, Guowang1
关键词Chemical Components Liquid Chromatography Mass Spectrometry Metabolic Profiling Tobacco Leaves
刊名JOURNAL OF SEPARATION SCIENCE
2011
DOI10.1002/jssc.201000652
34期:2页:119-126
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]MASS-SPECTROMETRY ; GAS-CHROMATOGRAPHY ; PLANT METABOLOMICS ; CROSS-VALIDATION ; IDENTIFICATION ; EXTRACTION ; STRATEGY ; BIOLOGY ; MODELS ; SMOKE
英文摘要DAn approach was developed for extracting and analyzing the chemical components of tobacco leaves based on solvent extraction and rapid & resolution liquid chromatography/quadrupole time-of-flight mass spectrometry analysis. Two solvents with different polarities were used to extract hydrophilic components and hydrophobic components, respectively, the combined analytical data can provide a "global'' view of metabolites. Based on the evaluation of parallel samples, it was found that this approach provided good repeatability, accurate and reliable profiling data, and is suitable for the metabolomics study of tobacco leaves. In order to find the chemical component differences of tobacco leaves, 56 samples from Zimbabwe and China were analyzed using the developed method. The metabolite data were processed by multivariate statistic technique; an obvious group classification between Zimbabwe and China was observed, 14 significantly changed compounds were found, and 9 of them were identified.
语种英语
WOS记录号WOS:000287603400001
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/142477
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Hongyun Tobacco Grp Co Ltd, Kunming Cigarette Factory, Kunming, Peoples R China
3.Yunnan Acad Tobacco Agr Sci, Yuxi, Peoples R China
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GB/T 7714
Li, Qinghua,Zhao, Chunxia,Li, Yong,et al. Liquid chromatography/mass spectrometry-based metabolic profiling to elucidate chemical differences of tobacco leaves between Zimbabwe and China[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(2):119-126.
APA Li, Qinghua.,Zhao, Chunxia.,Li, Yong.,Chang, Yuwei.,Wu, Zeming.,...&Xu, Guowang.(2011).Liquid chromatography/mass spectrometry-based metabolic profiling to elucidate chemical differences of tobacco leaves between Zimbabwe and China.JOURNAL OF SEPARATION SCIENCE,34(2),119-126.
MLA Li, Qinghua,et al."Liquid chromatography/mass spectrometry-based metabolic profiling to elucidate chemical differences of tobacco leaves between Zimbabwe and China".JOURNAL OF SEPARATION SCIENCE 34.2(2011):119-126.
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