中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Rapid and sensitive detection of DNA polymerase fidelity by singly labeled smart fluorescent probes
作者: Song, Chen1;  Zhang, Chen1;  Zhao, Meiping1
关键词: DNA polymerase fidelity ;  Singly labeled smart fluorescent probe ;  Real-time monitoring ;  Mispair extension ;  Proofreading activity
刊名: BIOSENSORS & BIOELECTRONICS
发表日期: 2011-01-15
DOI: 10.1016/j.bios.2010.08.073
卷: 26, 期:5, 页:2699-2702
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry, Analytical ;  Electrochemistry ;  Nanoscience & Nanotechnology
研究领域[WOS]: Biophysics ;  Biotechnology & Applied Microbiology ;  Chemistry ;  Electrochemistry ;  Science & Technology - Other Topics
英文摘要: We report here a novel approach to monitor the DNA polymerase fidelity in detailed steps, including mispair extension, mispair formation and 3' -> 5' proofreading. The method is based on the photo-induced electron transfer between the natural base guanine and the labeled fluorophore. The G:T mispair extension catalyzed by the exonuclease-deficient Klenow fragment DNA polymerase (KF exo(-)) was easily detected and the effect of the nearest neighbor base pair on the mispair extension rate was clearly observed. More importantly, kinetics of the G:T, G:A and G:G mispair formation and extension under single turnover conditions were measured by continuous fluorescence-based assay for the first time. The probes also showed their applicability to discriminate the 3' -> 5' proofreading activity of different exonuclease-proficient DNA polymerases. The presented method may greatly simplify the screening and characterization procedures of the increasing number of polymerases that are thought to be potential targets for drug design and cancer treatment. It will also provide important information for deep understanding of the polymerase fidelity mechanism. (C) 2010 Elsevier B.V. All rights reserved.
关键词[WOS]: REPLICATION FIDELITY ;  MISPAIR EXTENSION ;  BIOCHEMICAL BASIS ;  ESCHERICHIA-COLI ;  KLENOW FRAGMENT ;  WILD-TYPE ;  BASE ;  2-AMINOPURINE ;  BACTERIOPHAGE-T4 ;  NUCLEOTIDES
语种: 英语
WOS记录号: WOS:000286904400149
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142479
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Coll Chem & Mol Engn, Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci,Minist Educ, Beijing 100871, Peoples R China

Recommended Citation:
Song, Chen,Zhang, Chen,Zhao, Meiping. Rapid and sensitive detection of DNA polymerase fidelity by singly labeled smart fluorescent probes[J]. BIOSENSORS & BIOELECTRONICS,2011,26(5):2699-2702.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Song, Chen]'s Articles
 [Zhang, Chen]'s Articles
 [Zhao, Meiping]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Song, Chen]‘s Articles
 [Zhang, Chen]‘s Articles
 [Zhao, Meiping]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace