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题名: Proteomic reactors and their applications in biology
作者: Zhou, Hu1, 2, 3;  Ning, Zhibin1, 3;  Wang, Fangjun1, 3, 4;  Seebun, Deeptee1, 3;  Figeys, Daniel1, 3
关键词: fractionation ;  immobilized enzyme reactor ;  liquid chromatography ;  mass spectrometry ;  microfluidic ;  post-translational modification ;  proteomic reactor ;  proteomics ;  strong anion exchange ;  strong cation exchange
刊名: FEBS JOURNAL
发表日期: 2011-10-01
DOI: 10.1111/j.1742-4658.2011.08292.x
卷: 278, 期:20, 页:3796-3806
收录类别: SCI
文章类型: Review
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Proteomic analysis requires the combination of an extensive suite of technologies including protein processing and separation, micro-flow HPLC, MS and bioinformatics. Although proteomic technologies are still in flux, approaches that bypass gel electrophoresis (gel-free approaches) are dominating the field of proteomics. Along with the development of gel-free proteomics, came the development of devices for the processing of proteomic samples termed proteomic reactors. These microfluidic devices provide rapid, robust and efficient pre-MS sample procession by performing protein sample preparation/concentration, digestion and peptide fractionation. The proteomic reactor has advanced in two major directions: immobilized enzyme reactor and ion exchange-based proteomic reactor. This review summarizes the technical developments and biological applications of the proteomic reactor over the last decade.
关键词[WOS]: STRONG CATION-EXCHANGE ;  HIGHLY EFFICIENT PROTEOLYSIS ;  TANDEM MASS-SPECTROMETRY ;  PROTEIN IDENTIFICATION ;  LIQUID-CHROMATOGRAPHY ;  ELECTROSPRAY-IONIZATION ;  MICROFLUIDIC REACTOR ;  PEPTIDE SEPARATION ;  MEMBRANE PROTEOME ;  N-GLYCOPROTEOME
语种: 英语
WOS记录号: WOS:000295335500002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142505
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Univ Ottawa, Dept Biochem Microbiol & Immunol, Ottawa, ON K1H 8M5, Canada
2.Chinese Acad Sci, Shanghai Inst Mat Med, Beijing 100864, Peoples R China
3.Univ Ottawa, OISB, Ottawa, ON K1H 8M5, Canada
4.Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China

Recommended Citation:
Zhou, Hu,Ning, Zhibin,Wang, Fangjun,et al. Proteomic reactors and their applications in biology[J]. FEBS JOURNAL,2011,278(20):3796-3806.
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