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Proteomic reactors and their applications in biology
Zhou, Hu1,2,3; Ning, Zhibin1,3; Wang, Fangjun1,3,4; Seebun, Deeptee1,3; Figeys, Daniel1,3
关键词Fractionation Immobilized Enzyme Reactor Liquid Chromatography Mass Spectrometry Microfluidic Post-translational Modification Proteomic Reactor Proteomics Strong Anion Exchange Strong Cation Exchange
刊名FEBS JOURNAL
2011-10-01
DOI10.1111/j.1742-4658.2011.08292.x
278期:20页:3796-3806
收录类别SCI
文章类型Review
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]STRONG CATION-EXCHANGE ; HIGHLY EFFICIENT PROTEOLYSIS ; TANDEM MASS-SPECTROMETRY ; PROTEIN IDENTIFICATION ; LIQUID-CHROMATOGRAPHY ; ELECTROSPRAY-IONIZATION ; MICROFLUIDIC REACTOR ; PEPTIDE SEPARATION ; MEMBRANE PROTEOME ; N-GLYCOPROTEOME
英文摘要Proteomic analysis requires the combination of an extensive suite of technologies including protein processing and separation, micro-flow HPLC, MS and bioinformatics. Although proteomic technologies are still in flux, approaches that bypass gel electrophoresis (gel-free approaches) are dominating the field of proteomics. Along with the development of gel-free proteomics, came the development of devices for the processing of proteomic samples termed proteomic reactors. These microfluidic devices provide rapid, robust and efficient pre-MS sample procession by performing protein sample preparation/concentration, digestion and peptide fractionation. The proteomic reactor has advanced in two major directions: immobilized enzyme reactor and ion exchange-based proteomic reactor. This review summarizes the technical developments and biological applications of the proteomic reactor over the last decade.
语种英语
WOS记录号WOS:000295335500002
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被引频次:29[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/142505
专题中国科学院大连化学物理研究所
作者单位1.Univ Ottawa, Dept Biochem Microbiol & Immunol, Ottawa, ON K1H 8M5, Canada
2.Chinese Acad Sci, Shanghai Inst Mat Med, Beijing 100864, Peoples R China
3.Univ Ottawa, OISB, Ottawa, ON K1H 8M5, Canada
4.Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
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Zhou, Hu,Ning, Zhibin,Wang, Fangjun,et al. Proteomic reactors and their applications in biology[J]. FEBS JOURNAL,2011,278(20):3796-3806.
APA Zhou, Hu,Ning, Zhibin,Wang, Fangjun,Seebun, Deeptee,&Figeys, Daniel.(2011).Proteomic reactors and their applications in biology.FEBS JOURNAL,278(20),3796-3806.
MLA Zhou, Hu,et al."Proteomic reactors and their applications in biology".FEBS JOURNAL 278.20(2011):3796-3806.
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