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题名: Isolation and quantitative determination of inulin-type oligosaccharides in roots of Morinda officinalis
作者: Yang, Zhenmin1, 2;  Hu, Jun1;  Zhao, Mingyue1
关键词: Inulin-type oligosaccharides ;  Isolation ;  Quantitative determination ;  HPLC-ELSD ;  Cyclodextrin-bond column
刊名: CARBOHYDRATE POLYMERS
发表日期: 2011-02-01
DOI: 10.1016/j.carbpol.2010.11.006
卷: 83, 期:4, 页:1997-2004
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Applied ;  Chemistry, Organic ;  Polymer Science
研究领域[WOS]: Chemistry ;  Polymer Science
英文摘要: Inulin-type oligosaccharides with different degree of polymerization (DP) were isolated from the traditional Chinese medicine: Morina officinalis by size-exclusion chromatography, and their purities were determined by HPLC-ELSD equipped with cyclodextrin-bond column. Through analysis, the purities of obtained inulin-type oligosaccharides were higher more than 98% by one-step process. The structures of inulin-type oligosaccharides were confirmed by a combination of NMR, MS as well as comparison with already existing NMR data. Using D-fructose and the isolated inulin-type oligosaccharides as standards, a determination method of monosaccharide and inulin-type oligosaccharides in Morinda officinalis was first developed and validated. The validated method was successfully applied to analyze monosaccharide and oligosaccharides in three types of roots of M. officinalis and provided a new basis of assessment on quality of M. officinalis. (C) 2010 Elsevier Ltd. All rights reserved.
关键词[WOS]: CYCLODEXTRIN-BONDED PHASE ;  STRUCTURAL-CHARACTERIZATION ;  PC12 CELLS ;  MEDICINE ;  NMR ;  FRUCTOOLIGOSACCHARIDES ;  POLYSACCHARIDES ;  CORTICOSTERONE ;  IDENTIFICATION ;  TRISACCHARIDES
语种: 英语
WOS记录号: WOS:000286965400075
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142528
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Zhengzhou Tobacco Res Inst CNTC, Lab Flavor & Fragrance, Zhengzhou 450001, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Yang, Zhenmin,Hu, Jun,Zhao, Mingyue. Isolation and quantitative determination of inulin-type oligosaccharides in roots of Morinda officinalis[J]. CARBOHYDRATE POLYMERS,2011,83(4):1997-2004.
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