中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: A 3D cartilage - Inflammatory cell culture system for the modeling of human osteoarthritis
作者: Sun, Lin2;  Wang, Xiuli1, 3;  Kaplan, David L.1, 2
关键词: Osteoarthritis ;  Tissue engineered cartilage ;  Inflammation ;  Silk
刊名: BIOMATERIALS
发表日期: 2011-08-01
DOI: 10.1016/j.biomaterials.2011.04.028
卷: 32, 期:24, 页:5581-5589
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Engineering, Biomedical ;  Materials Science, Biomaterials
研究领域[WOS]: Engineering ;  Materials Science
英文摘要: Inflammation plays a major role in the destruction of cartilage in osteoarthritis (OA), with the interaction of multiple mediators, immune cells, fibroblasts and chondrocytes. Current 2D studies in vitro with cell lines, as well as animal models, are limited in terms of providing insight into pathogenic mechanisms related to the human system. Hence, an in vitro human 3D cartilage tissue system was established to study the impact of inflammatory mediators on chondrocytes and matrices as an initial approach to emulating early stages of OA. An in vitro 3D human cartilage tissue system was established by culturing primary chondrocytes in silk protein porous scaffolds up to 21 days in static culture, with and without cytokine (IL-1 beta and TNF-alpha) exposure or with the use of macrophage conditioned medium (MCM). To assess chondrocyte responses, transcript levels, histology and immunohistochemistry were used to assess changes in cell viability and in cartilage matrix composition, including collagen type II and aggrecan. Chondrocyte hypertrophy and apoptosis were assessed via collagen type X and caspase-3. RTPCR revealed that the cytokines and the MCM regulated matrix-related gene expression of chondrocytes. but with different outcomes. For anabolic-encoding genes, MCM suppressed collagen type II and upregulated aggrecan. In contrast, the cytokines suppressed aggrecan formation and had no effect on collagen type II. For catabolic-encoded genes, both cytokines and MCM upregulated MMP1, MMP3, MMP13 and ADAMTS4, with cytokines preferentially upregulating MMP13 and MCM upregulating ADMTS4. MCM down-regulated ADAMTS5. In addition, MCM stimulation led to hypertrophy and apoptosis of chondrocytes, outcomes not found with the cytokine treatment group. A decrease in aggrecan content with cytokines and MCM stimulation was found, while MCM resulted in greater reduction than the cytokine treatment. The results demonstrated that OA-like features, such as changes in matrix synthesis gene expression, increase of collagense gene expression and loss of aggrecan, were initiated within this 3D chrondrocyte human tissue system upon stimulation of the cultures with cytokines and MCM. MCM was a better inducer of immune-related features of OA, because besides the features found with cytokine stimulation, the MCM treatment also initiated collagen X expression and deposition and apoptosis of chondrocytes, important features of human OA. The results obtained with this new in vitro tissue model provide an initial step towards the development of an early stage OA system to allow for more systematic study and insight into the origins and outcomes with this disease. (C) 2011 Elsevier Ltd. All rights reserved.
关键词[WOS]: TUMOR-NECROSIS-FACTOR ;  HUMAN ARTICULAR CHONDROCYTES ;  COLLAGEN GENE-EXPRESSION ;  NITRIC-OXIDE ;  FACTOR-ALPHA ;  MATRIX METALLOPROTEINASES ;  OSTEOPHYTE FORMATION ;  PROSTAGLANDIN E-2 ;  INTERFERON-GAMMA ;  SILK SCAFFOLDS
语种: 英语
WOS记录号: WOS:000292431100006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142537
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Tufts Univ, Dept Biomed Engn, Medford, MA 02155 USA
2.Tufts Univ, Dept Chem & Biol Engn, Medford, MA 02155 USA
3.Chinese Acad Sci, Dalian Inst Chem & Phys, Dalian 116023, Peoples R China

Recommended Citation:
Sun, Lin,Wang, Xiuli,Kaplan, David L.. A 3D cartilage - Inflammatory cell culture system for the modeling of human osteoarthritis[J]. BIOMATERIALS,2011,32(24):5581-5589.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Sun, Lin]'s Articles
 [Wang, Xiuli]'s Articles
 [Kaplan, David L.]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Sun, Lin]‘s Articles
 [Wang, Xiuli]‘s Articles
 [Kaplan, David L.]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace