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题名: Ionic liquid-assisted SDS-PAGE to improve human serum protein separation
作者: Zhang, Tao1;  Gai, Qingqing1;  Qu, Feng1;  Zhang, Yukui2
关键词: Human serum ;  ILs-SDS-PAGE ;  Ionic liquids ;  Protein separation
刊名: ELECTROPHORESIS
发表日期: 2011-10-01
DOI: 10.1002/elps.201100184
卷: 32, 期:20, 页:2904-2910
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Ionic liquid (IL)-assisted sodium dodecyl sulfate polyacrylamide gel electrophoresis (ILs-SDS-PAGE) was presented to improve protein separation. ILs were employed during the preparation process of polyacrylamide gel, then the modified gel was used for commercial protein marker, binary bovine serum albumin/lysozyme (BSA/Lyz) and human serum separation. The influence of ionic liquid concentration, cation alkyl chain length, cation and anion types on proteins separation were investigated. The results showed that ILs played a role in improving some protein separation, and ILs-SDS-PAGE provided higher resolution and separation efficiency than ordinary SDS-PAGE for low and middle relative molecular mass proteins in human serum. In addition, the principle of ILs-SDS-PAGE was discussed and the comparison of ILs-SDS-PAGE with ordinary SDS-PAGE and Native PAGE was made.
关键词[WOS]: POLYACRYLAMIDE-GEL ELECTROPHORESIS ;  BLUE NATIVE ELECTROPHORESIS ;  CAPILLARY-ELECTROPHORESIS ;  MOLECULAR-WEIGHT ;  MASS-SPECTROMETRY ;  BASIC-PROTEINS ;  2-D PAGE ;  PROTEOMICS ;  CHROMATOGRAPHY ;  ELECTROLYTE
语种: 英语
WOS记录号: WOS:000297424500018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142541
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China

Recommended Citation:
Zhang, Tao,Gai, Qingqing,Qu, Feng,et al. Ionic liquid-assisted SDS-PAGE to improve human serum protein separation[J]. ELECTROPHORESIS,2011,32(20):2904-2910.
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