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题名: Star-shaped polymers for DNA sequencing by capillary electrophoresis
作者: Gao, Fan;  Tie, Cal;  Zhang, Xin-Xiang1;  Niu, Zhiqiang;  He, Xiaojin;  Ma, Yuguo
关键词: PDMA ;  Star-shaped polymer ;  DNA sieving ;  Capillary electrophoresis
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2011-05-20
DOI: 10.1016/j.chroma.2011.03.027
卷: 1218, 期:20, 页:3037-3041
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The separation and sequencing of DNA are the main objectives of the Human Genome Project, and this project has also been very useful for gene analysis and disease diagnosis. Capillary electrophoresis (CE) is one of the most common techniques for the separation and analysis of DNA. DNA separations are usually achieved using capillary gel electrophoresis (CGE) mode, in which polymer gel is packed into the capillary. Compared with a traditional CGE matrix, a hydrophilic polymer matrix, which can be adsorb by the capillary wall has numerous advantages, including stability, reproducibility and ease of automation. Various water-soluble additives, such as linear poly(acrylamide) (PAA) and poly(N,N-dimethylacrylamide) (PDMA), have been employed as media. In this study, different star-shaped PDMA polymers were designed and synthesized to achieve lower polymer solution viscosity. DNA separations with these polymers avoid the disadvantages of high viscosity and long separation time while maintaining high resolution (10 bp between 271 bp and 281 bp). The influences of the polymer concentration and structure on DNA separation were also determined in this study; higher polymer concentration yielded better separation performance, and star-like polymers were superior to linear polymers. This work indicates that modification of the polymer structure is a potential strategy for optimizing DNA separation. (C) 2011 Elsevier B.V. All rights reserved.
关键词[WOS]: IMPROVED HYDROLYTIC STABILITY ;  LOW-VISCOSITY POLYMER ;  POLY(VINYLPYRROLIDONE) SOLUTION ;  HIGHER HYDROPHILICITY ;  POLY(ETHYLENE OXIDE) ;  ACRYLAMIDO MONOMERS ;  SEPARATION CHIPS ;  FRAGMENTS ;  POLYACRYLAMIDES ;  MUTATIONS
语种: 英语
WOS记录号: WOS:000291065500027
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142571
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Peking Univ, Coll Chem, Key Lab Polymer Chem & Phys, Minist Educ,BNLMS, Beijing 100871, Peoples R China
2.Peking Univ, Coll Chem, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,BNLMS, Beijing 100871, Peoples R China

Recommended Citation:
Gao, Fan,Tie, Cal,Zhang, Xin-Xiang,et al. Star-shaped polymers for DNA sequencing by capillary electrophoresis[J]. JOURNAL OF CHROMATOGRAPHY A,2011,1218(20):3037-3041.
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