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题名: Octyl-functionalized hybrid magnetic mesoporous microspheres for enrichment of low-concentration peptides prior to direct analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
作者: Sun, Liangliang1, 2;  Zhao, Qun1, 2;  Zhu, Guijie1;  Zhou, Yuan1, 2;  Wang, Tingting1, 2;  Shan, Yichu1;  Yang, Kaiguang1;  Liang, Zhen1;  Zhang, Lihua1;  Zhang, Yukui1
刊名: RAPID COMMUNICATIONS IN MASS SPECTROMETRY
发表日期: 2011-05-15
DOI: 10.1002/rcm.4986
卷: 25, 期:9, 页:1257-1265
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences ;  Technology
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical ;  Spectroscopy
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry ;  Spectroscopy
英文摘要: Octyl-functionalized hybrid magnetic mesoporous (Fe(3)O(4)center dot nSiO(2)center dot meso-hybrid-C8) microspheres were synthesized and applied in the isolation and pre-concentration of low-concentration peptides prior to direct analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Such microspheres possess high surface area (324 m(2)/g), hydrophobic group (C8), relatively large pore volume (0.304 cm(3)/g), uniform pore diameter (similar to 3.7 nm), and magnetic responsivity, which make them a simple and efficient kind of adsorbent for the enrichment of low-concentration peptides. For bovine serum albumin (BSA, 15 fmol mu L(-1)) digest, after concentration by Fe(3)O(4)center dot nSiO(2)center dot meso-hybrid-C8 microspheres, the enrichment performance was evidently better than those obtained by solvent evaporation and C8-functionalized magnetic particles, and comparable to those obtained by commercial Anchor chip target and ZipTipC18 pipette tip. Such microspheres were further applied in the enrichment of the tryptic digests of rat cerebellum proteins and endogenous peptides of crude human serum, and more peaks with higher signal-to-noise (S/N) ratio were obtained than before pre-concentration. Furthermore, the pre-concentration reproducibility of magnetic microspheres for biological samples was good, and the limit of detection (LOD) for BSA digests by MALDI-TOF MS was decreased by at least one order of magnitude compared with that obtained without pre-concentration. All the above-mentioned results indicate that the synthesized Fe(3)O(4)center dot nSiO(2)center dot meso-hybrid-C8 microspheres are promising for the enrichment of low-concentration peptides from complex biosamples. Copyright (C) 2011 John Wiley & Sons, Ltd.
关键词[WOS]: TOF-MS ANALYSIS ;  LOW-ABUNDANCE PEPTIDES ;  CAPILLARY ELECTROCHROMATOGRAPHY ;  HIGHLY EFFICIENT ;  MONOLITHIC COLUMN ;  SILICA PARTICLES ;  HUMAN PLASMA ;  SIO2 SHELL ;  PROTEINS ;  NANOPARTICLES
语种: 英语
WOS记录号: WOS:000290452600017
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142573
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Sun, Liangliang,Zhao, Qun,Zhu, Guijie,et al. Octyl-functionalized hybrid magnetic mesoporous microspheres for enrichment of low-concentration peptides prior to direct analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2011,25(9):1257-1265.
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