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题名: A selenium-dependent glutathione peroxidase in the Japanese scallop, Mizuhopecten yessoensis: cDNA cloning, promoter sequence analysis and mRNA expression
作者: Shan, Zhongguo2;  Li, Hongjun1, 3;  Bao, Xiangbo1, 3;  He, Chongbo1, 3;  Yu, Henan2;  Liu, Weidong1, 3, 4;  Hou, Lin2;  Wang, Juan2;  Zhu, Dan2;  Sui, Lijun2;  Zhu, Bao2;  Li, Yunfeng2, 3
关键词: Mizuhopecten yessoensis ;  Selenium-dependent glutathione peroxidase ;  mRNA expression ;  Promoter analysis ;  SNP
刊名: COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
发表日期: 2011-05-01
DOI: 10.1016/j.cbpb.2011.01.003
卷: 159, 期:1, 页:1-9
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biochemistry & Molecular Biology ;  Zoology
研究领域[WOS]: Biochemistry & Molecular Biology ;  Zoology
英文摘要: Glutathione peroxidase (GPx) is an antioxidant enzyme that protects cells from oxidative damage in the innate immune responses against bacterial infections. GPx is also involved in immune defenses. In this study, we report cloning and characterization of a GPx (designated as MyGPx) coding sequences and promoter from Japanese scallop, Mizuhopecten yessoensis. The full-length 1081 nt MyGPx mRNA contained a 28 nt 5' untranslated region (UTR), a 603 nt open reading frame and a 450 nt 3' UTR containing a polyadenylation signal (AATAAA). Multiple sequence alignment revealed that amino acids essential to enzymatic function of MyGPx proteins were highly conserved. A 1628 nt 5'-flanking sequence of MyGPx was identified by genome walking. Here, several potential transcription factor binding sites were detected in the putative promoter region, and nine single nucleotide polymorphisms (SNPs) were found in the 5' sequence flanking the promoter region. Quantitative Real time PCR (qRT-PCR) was employed to measure GPx mRNA expression in adult tissues and monitor mRNA expression patterns during embryonic development and following stimulation by the bacteria Vibrillo anguillarum. Collectively, the results suggest that MyGPx fulfills an important function during M. yessoensis development and may be an important immune effector in adult molluscs. (C) 2011 Elsevier Inc. All rights reserved.
关键词[WOS]: MOLLUSK IMMUNE CELLS ;  TRANSCRIPTION FACTOR ;  ANTIOXIDANT ENZYME ;  SPERM MATURATION ;  BINDING-ACTIVITY ;  REACTIVE OXYGEN ;  GENE ;  SELENOCYSTEINE ;  IDENTIFICATION ;  SELENOENZYME
语种: 英语
WOS记录号: WOS:000289448600001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142582
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Liaoning Ocean & Fisheries Sci Res Inst, Dalian 116023, Peoples R China
2.Liaoning Normal Univ, Coll Life Sci, Dalian 116029, Peoples R China
3.Key Lab Marine Fishery Mol Biol, Dalian 116023, Peoples R China
4.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

Recommended Citation:
Shan, Zhongguo,Li, Hongjun,Bao, Xiangbo,et al. A selenium-dependent glutathione peroxidase in the Japanese scallop, Mizuhopecten yessoensis: cDNA cloning, promoter sequence analysis and mRNA expression[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2011,159(1):1-9.
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