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题名: Preparation and characterization of uniformly sized molecularly imprinted polymers functionalized with core-shell magnetic nanoparticles for the recognition and enrichment of protein
作者: Gao, Ruixia1;  Kong, Xuan1;  Wang, Xin1;  He, Xiwen1;  Chen, Langxing1;  Zhang, Yukui1, 2
刊名: JOURNAL OF MATERIALS CHEMISTRY
发表日期: 2011
DOI: 10.1039/c1jm12414e
卷: 21, 期:44, 页:17863-17871
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Physical ;  Materials Science, Multidisciplinary
研究领域[WOS]: Chemistry ;  Materials Science
英文摘要: A general method to prepare thin, molecularly imprinted polymer (MIP) coatings on magnetic Fe(3)O(4) nanoparticles (NPs) with a uniform core-shell structure for the recognition and enrichment of protein was developed. Four proteins (bovine serum albumin (BSA, pI = 4.9), bovine hemoglobin (BHb, pI = 6.9), bovine pancreas ribonuclease A (RNase A, pI = 9.4) and lysozyme (Lyz, pI = 11.2)) with different isoelectric points were chosen as the templates. The magnetic protein-MIPs were synthesized by combining surface imprinting and sol-gel techniques. The morphology, adsorption and recognition properties of the magnetic molecularly imprinted NPs were investigated by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared (FT-IR) spectroscopy and through the use of a vibrating sample magnetometer (VSM). In comparison with the use of Lyz, BSA and RNase A as template proteins, BHb-imprinted Fe(3)O(4) showed the best imprinting effect and the highest adsorption capacity among the four proteins. The as-prepared Fe(3)O(4)@ BHb-MIPs NPs with a mean diameter of 230 nm were coated with an MIP shell that was 10 nm thick, which enabled the Fe(3)O(4)@BHb-MIPs to easily reach adsorption equilibrium. A high magnetic saturation value of 25.47 emu g(-1) for Fe(3)O(4)@BHb-MIPs NPs was obtained, which endowed the adsorbent with the convenience of magnetic separation under an external magnetic field. The resultant Fe(3)O(4)@BHb-MIPs NPs could not only selectively extract a target protein from mixed proteins but also specifically capture the protein BHb from a real sample of bovine blood. In addition, different batches of magnetic MIPs showed good reproducibility and reusability for at least six repeated cycles.
关键词[WOS]: TOF-MS ANALYSIS ;  IRON-OXIDE NANOPARTICLES ;  LOW-CONCENTRATION PEPTIDES ;  HISTIDINE-TAGGED PROTEINS ;  BOVINE HEMOGLOBIN ;  FACILE SYNTHESIS ;  BIOMEDICAL APPLICATIONS ;  SILICA MICROSPHERES ;  HIGHLY EFFICIENT ;  PARTICLES
语种: 英语
WOS记录号: WOS:000296390600047
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142613
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China

Recommended Citation:
Gao, Ruixia,Kong, Xuan,Wang, Xin,et al. Preparation and characterization of uniformly sized molecularly imprinted polymers functionalized with core-shell magnetic nanoparticles for the recognition and enrichment of protein[J]. JOURNAL OF MATERIALS CHEMISTRY,2011,21(44):17863-17871.
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