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题名: High-performance purification of quaternary alkaloids from Corydalis yanhusuo W. T. Wang using a new polar-copolymerized stationary phase
作者: Wang, Chaoran1;  Guo, Zhimou1;  Zhang, Jing1;  Zeng, Jing1;  Zhang, Xiuli1;  Liang, Xinmiao1
关键词: Alkaloids ;  Corydalis yanhusuo W. T. Wang ;  HPLC ;  Polar-copolymerized ;  Purification
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2011
DOI: 10.1002/jssc.201000625
卷: 34, 期:1, 页:53-58
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: An efficient method for purification of alkaloids from Corydalis yanhusuo W. T. Wang using HPLC was developed, featuring a polar-copolymerized stationary phase named C18HCE. As ionizable solutes, the crude alkaloid sample often suffered from serious peak tailing problem on conventional RP-LC columns, and the separation would rapidly became destroyed with the increasing of load amount. However, on the new stationary phase, good peak shapes (asymmetry factor <1.5) as well as good loadability were easily obtained in a commonly used acidic mobile phase condition. The loading amount could reach 10 mg per injection on an analytical C18HCE column for laboratory-scale purification. About 6.8 mg of palmatine (HPLC purity >98%) and 44.4 mg of dehydrocorydaline (H PLC purity >98%) were rapidly derived from 200 mg of the crude alkaloid sample, and the recoveries of these two compounds were 76.5 and 81.7%, respectively. The purified alkaloids were characterized by comparing retention times with standard compounds as well as (1)H-NMR data. The new method is simple and high yielding, and it may provide a promising tool for purification of alkaloids as well as other alkaline compounds.
关键词[WOS]: LIQUID-CHROMATOGRAPHY ;  BASIC COMPOUNDS ;  PH ;  SEPARATIONS ;  RETENTION ;  SAXICOLA ;  COLUMNS ;  BASES ;  HPLC
语种: 英语
WOS记录号: WOS:000286452700008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142619
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Key Lab Nat Med, Dalian 116023, Liaoning Prov, Peoples R China

Recommended Citation:
Wang, Chaoran,Guo, Zhimou,Zhang, Jing,et al. High-performance purification of quaternary alkaloids from Corydalis yanhusuo W. T. Wang using a new polar-copolymerized stationary phase[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(1):53-58.
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