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High-performance purification of quaternary alkaloids from Corydalis yanhusuo W. T. Wang using a new polar-copolymerized stationary phase
Wang, Chaoran; Guo, Zhimou; Zhang, Jing; Zeng, Jing; Zhang, Xiuli; Liang, Xinmiao
关键词Alkaloids Corydalis Yanhusuo W. T. W.ng Hplc Polar-copolymerized Purification
刊名JOURNAL OF SEPARATION SCIENCE
2011
DOI10.1002/jssc.201000625
34期:1页:53-58
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]LIQUID-CHROMATOGRAPHY ; BASIC COMPOUNDS ; PH ; SEPARATIONS ; RETENTION ; SAXICOLA ; COLUMNS ; BASES ; HPLC
英文摘要An efficient method for purification of alkaloids from Corydalis yanhusuo W. T. Wang using HPLC was developed, featuring a polar-copolymerized stationary phase named C18HCE. As ionizable solutes, the crude alkaloid sample often suffered from serious peak tailing problem on conventional RP-LC columns, and the separation would rapidly became destroyed with the increasing of load amount. However, on the new stationary phase, good peak shapes (asymmetry factor <1.5) as well as good loadability were easily obtained in a commonly used acidic mobile phase condition. The loading amount could reach 10 mg per injection on an analytical C18HCE column for laboratory-scale purification. About 6.8 mg of palmatine (HPLC purity >98%) and 44.4 mg of dehydrocorydaline (H PLC purity >98%) were rapidly derived from 200 mg of the crude alkaloid sample, and the recoveries of these two compounds were 76.5 and 81.7%, respectively. The purified alkaloids were characterized by comparing retention times with standard compounds as well as (1)H-NMR data. The new method is simple and high yielding, and it may provide a promising tool for purification of alkaloids as well as other alkaline compounds.
语种英语
WOS记录号WOS:000286452700008
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被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/142619
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Key Lab Nat Med, Dalian 116023, Liaoning Prov, Peoples R China
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Wang, Chaoran,Guo, Zhimou,Zhang, Jing,et al. High-performance purification of quaternary alkaloids from Corydalis yanhusuo W. T. Wang using a new polar-copolymerized stationary phase[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(1):53-58.
APA Wang, Chaoran,Guo, Zhimou,Zhang, Jing,Zeng, Jing,Zhang, Xiuli,&Liang, Xinmiao.(2011).High-performance purification of quaternary alkaloids from Corydalis yanhusuo W. T. Wang using a new polar-copolymerized stationary phase.JOURNAL OF SEPARATION SCIENCE,34(1),53-58.
MLA Wang, Chaoran,et al."High-performance purification of quaternary alkaloids from Corydalis yanhusuo W. T. Wang using a new polar-copolymerized stationary phase".JOURNAL OF SEPARATION SCIENCE 34.1(2011):53-58.
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