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Simultaneous discrimination of jasmonic acid stereoisomers by CE-QTOF-MS employing the partial filling technique
Han, Yehua1; Bai, Yu1; Xiao, Yiling1; Du, Fuyou1; Liang, Yuan1; Tan, Zhijing2; Zhao, Meiping1; Liu, Huwei1
关键词Ce-qtof-ms Chiral Separation Jasmonic Acid Partial Filling Technique Short-end Injection
刊名ELECTROPHORESIS
2011-10-01
DOI10.1002/elps.201100043
32期:19页:2693-2699
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]ELECTROPHORESIS-MASS-SPECTROMETRY ; CHITOSAN-COATED CAPILLARY ; IONIC POLYMER LAYERS ; ELECTROKINETIC CHROMATOGRAPHY ; LIQUID-CHROMATOGRAPHY ; METHYL JASMONATE ; MODIFIED CYCLODEXTRINS ; CHIRAL SEPARATION ; RESPONSES ; ISOMERS
英文摘要Jasmonic acid (JA), an essential plant hormone controlling the plant defense signaling system and developmental processes, has stereospecific bioactivities that have not been well understood mainly due to the limitation in separation and detection methodologies. In this work, a fast CE-UV method based on short-end injection technique and a sensitive CE-QTOF-MS method based on partial filling technique were successfully developed for the enantioseparation of racemic JA. The successive coating technique was also involved by modifying the capillary with multiple ionic polymer layers of polybrene-dextran sulfate-polybrene. This was the first report on the direct resolution of both pairs of JA enantiomers, including two naturally occurring JA stereoisomers. Although no pure JA stereoisomers were commercially available, all the separated JA stereoisomers were identified indirectly by comparing the difference between the racemic standard and plant samples based on the presence and the ratio of each stereoisomer. Satisfactory results were obtained in terms of sensitivity (LOD, 24 ng/mL or 0.7 fmol for single JA stereoisomer) using 45 mmol/L ammonium acetate at pH 4.5 containing 70 mmol/L alpha-CD as the buffer system. This established CE-QTOF-MS method was later successfully applied for the study of the naturally occurring JA stereoisomers in wounded tobacco leaves.
语种英语
WOS记录号WOS:000296606000012
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被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/142627
专题中国科学院大连化学物理研究所
作者单位1.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Inst Analyt Chem,Coll Chem & Mol Engn, Beijing 100871, Peoples R China
2.Peking Univ, Natl Key Lab Prot Engn & Plant Genet Engn, Coll Life Sci, Beijing 100871, Peoples R China
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Han, Yehua,Bai, Yu,Xiao, Yiling,et al. Simultaneous discrimination of jasmonic acid stereoisomers by CE-QTOF-MS employing the partial filling technique[J]. ELECTROPHORESIS,2011,32(19):2693-2699.
APA Han, Yehua.,Bai, Yu.,Xiao, Yiling.,Du, Fuyou.,Liang, Yuan.,...&Liu, Huwei.(2011).Simultaneous discrimination of jasmonic acid stereoisomers by CE-QTOF-MS employing the partial filling technique.ELECTROPHORESIS,32(19),2693-2699.
MLA Han, Yehua,et al."Simultaneous discrimination of jasmonic acid stereoisomers by CE-QTOF-MS employing the partial filling technique".ELECTROPHORESIS 32.19(2011):2693-2699.
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