DICP OpenIR
Simultaneous discrimination of jasmonic acid stereoisomers by CE-QTOF-MS employing the partial filling technique
Han, Yehua1; Bai, Yu1; Xiao, Yiling1; Du, Fuyou1; Liang, Yuan1; Tan, Zhijing2; Zhao, Meiping1; Liu, Huwei1
KeywordCe-qtof-ms Chiral Separation Jasmonic Acid Partial Filling Technique Short-end Injection
Source PublicationELECTROPHORESIS
2011-10-01
DOI10.1002/elps.201100043
Volume32Issue:19Pages:2693-2699
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordELECTROPHORESIS-MASS-SPECTROMETRY ; CHITOSAN-COATED CAPILLARY ; IONIC POLYMER LAYERS ; ELECTROKINETIC CHROMATOGRAPHY ; LIQUID-CHROMATOGRAPHY ; METHYL JASMONATE ; MODIFIED CYCLODEXTRINS ; CHIRAL SEPARATION ; RESPONSES ; ISOMERS
AbstractJasmonic acid (JA), an essential plant hormone controlling the plant defense signaling system and developmental processes, has stereospecific bioactivities that have not been well understood mainly due to the limitation in separation and detection methodologies. In this work, a fast CE-UV method based on short-end injection technique and a sensitive CE-QTOF-MS method based on partial filling technique were successfully developed for the enantioseparation of racemic JA. The successive coating technique was also involved by modifying the capillary with multiple ionic polymer layers of polybrene-dextran sulfate-polybrene. This was the first report on the direct resolution of both pairs of JA enantiomers, including two naturally occurring JA stereoisomers. Although no pure JA stereoisomers were commercially available, all the separated JA stereoisomers were identified indirectly by comparing the difference between the racemic standard and plant samples based on the presence and the ratio of each stereoisomer. Satisfactory results were obtained in terms of sensitivity (LOD, 24 ng/mL or 0.7 fmol for single JA stereoisomer) using 45 mmol/L ammonium acetate at pH 4.5 containing 70 mmol/L alpha-CD as the buffer system. This established CE-QTOF-MS method was later successfully applied for the study of the naturally occurring JA stereoisomers in wounded tobacco leaves.
Language英语
WOS IDWOS:000296606000012
Citation statistics
Cited Times:17[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/142627
Collection中国科学院大连化学物理研究所
Affiliation1.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Inst Analyt Chem,Coll Chem & Mol Engn, Beijing 100871, Peoples R China
2.Peking Univ, Natl Key Lab Prot Engn & Plant Genet Engn, Coll Life Sci, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Han, Yehua,Bai, Yu,Xiao, Yiling,et al. Simultaneous discrimination of jasmonic acid stereoisomers by CE-QTOF-MS employing the partial filling technique[J]. ELECTROPHORESIS,2011,32(19):2693-2699.
APA Han, Yehua.,Bai, Yu.,Xiao, Yiling.,Du, Fuyou.,Liang, Yuan.,...&Liu, Huwei.(2011).Simultaneous discrimination of jasmonic acid stereoisomers by CE-QTOF-MS employing the partial filling technique.ELECTROPHORESIS,32(19),2693-2699.
MLA Han, Yehua,et al."Simultaneous discrimination of jasmonic acid stereoisomers by CE-QTOF-MS employing the partial filling technique".ELECTROPHORESIS 32.19(2011):2693-2699.
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