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题名: Simultaneous discrimination of jasmonic acid stereoisomers by CE-QTOF-MS employing the partial filling technique
作者: Han, Yehua1;  Bai, Yu1;  Xiao, Yiling1;  Du, Fuyou1;  Liang, Yuan1;  Tan, Zhijing2;  Zhao, Meiping1;  Liu, Huwei1
关键词: CE-QTOF-MS ;  Chiral separation ;  Jasmonic acid ;  Partial filling technique ;  Short-end injection
刊名: ELECTROPHORESIS
发表日期: 2011-10-01
DOI: 10.1002/elps.201100043
卷: 32, 期:19, 页:2693-2699
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Jasmonic acid (JA), an essential plant hormone controlling the plant defense signaling system and developmental processes, has stereospecific bioactivities that have not been well understood mainly due to the limitation in separation and detection methodologies. In this work, a fast CE-UV method based on short-end injection technique and a sensitive CE-QTOF-MS method based on partial filling technique were successfully developed for the enantioseparation of racemic JA. The successive coating technique was also involved by modifying the capillary with multiple ionic polymer layers of polybrene-dextran sulfate-polybrene. This was the first report on the direct resolution of both pairs of JA enantiomers, including two naturally occurring JA stereoisomers. Although no pure JA stereoisomers were commercially available, all the separated JA stereoisomers were identified indirectly by comparing the difference between the racemic standard and plant samples based on the presence and the ratio of each stereoisomer. Satisfactory results were obtained in terms of sensitivity (LOD, 24 ng/mL or 0.7 fmol for single JA stereoisomer) using 45 mmol/L ammonium acetate at pH 4.5 containing 70 mmol/L alpha-CD as the buffer system. This established CE-QTOF-MS method was later successfully applied for the study of the naturally occurring JA stereoisomers in wounded tobacco leaves.
关键词[WOS]: ELECTROPHORESIS-MASS-SPECTROMETRY ;  CHITOSAN-COATED CAPILLARY ;  IONIC POLYMER LAYERS ;  ELECTROKINETIC CHROMATOGRAPHY ;  LIQUID-CHROMATOGRAPHY ;  METHYL JASMONATE ;  MODIFIED CYCLODEXTRINS ;  CHIRAL SEPARATION ;  RESPONSES ;  ISOMERS
语种: 英语
WOS记录号: WOS:000296606000012
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142627
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Peking Univ, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Minist Educ,Inst Analyt Chem,Coll Chem & Mol Engn, Beijing 100871, Peoples R China
2.Peking Univ, Natl Key Lab Prot Engn & Plant Genet Engn, Coll Life Sci, Beijing 100871, Peoples R China

Recommended Citation:
Han, Yehua,Bai, Yu,Xiao, Yiling,et al. Simultaneous discrimination of jasmonic acid stereoisomers by CE-QTOF-MS employing the partial filling technique[J]. ELECTROPHORESIS,2011,32(19):2693-2699.
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