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题名: Improvement of reproducibility and sensitivity of CE analysis by using the capillary coated dynamically with carboxymethyl chitosan
作者: Liu, Yi1;  Fu, Xiaofang1;  Bai, Yu1;  Zhai, Maolin2;  Liao, Yiping1;  Liao, Jie3;  Liu, Huwei1
关键词: CE-MS ;  Carboxymethyl chitosan ;  Dynamic coating ;  Reproducibility ;  Sensitivity
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2011-03-01
DOI: 10.1007/s00216-011-4659-2
卷: 399, 期:8, 页:2821-2829
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Analysis reproducibility and detection sensitivity of capillary electrophoresis (CE) are often questioned by applied scientists, which has hindered its application as a routine method. To address these issues, a simple, precise, and reproducible dynamic coating method was developed by applying carboxymethyl chitosan (CMC) dynamic coating on fused silica capillary. The proposed coating was accomplished by simply rinsing the capillary with CMC solution for 1 min in between runs, with no regeneration procedure or buffer additives needed. Electroosmotic flow could be well controlled by adjusting the pH of background electrolyte, and the adsorption of analytes onto the capillary inner wall was effectively eliminated. The main parameters of the coating condition were optimized, and extensive applications of these CMC-dynamically coated capillaries in CE separations were then firmly confirmed. By using proteins, aristolochic acids, and inorganic anions as model analytes, the coating showed a good stability, high reproducibility, as well as improved sensitivity. Baseline separations could be obtained with high efficiency. The reduced adsorption was impressively effective for basic proteins, with an average plate number of 90,000/m for each protein, apart from the good resolution on the chromatogram. A high sensitive detection of alpha-lactalbumin was achieved with a limit of detection (S/N = 3) of 3.5 nM, and the number of theoretical plates was as high as 1,200,000/m. In addition, the combination of the CMC coating with nonaqueous CE and CE-mass spectrometry proved to be practical. All results showed that the CMC-dynamically coated capillary has special properties and obvious superiority over the uncoated ones for CE analysis.
关键词[WOS]: ELECTROPHORESIS-MASS SPECTROMETRY ;  ARISTOLOCHIC ACID-I ;  ZONE-ELECTROPHORESIS ;  PROTEIN SEPARATIONS ;  INORGANIC ANIONS ;  LIQUID-CHROMATOGRAPHY ;  ELECTROKINETIC CHROMATOGRAPHY ;  ELECTROOSMOTIC FLOW ;  SURFACTANT COATINGS ;  BASIC-PROTEINS
语种: 英语
WOS记录号: WOS:000287576700022
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142633
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Peking Univ, Coll Chem & Mol Engn, Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci,Minist Educ, Beijing 100871, Peoples R China
2.Peking Univ, Coll Chem & Mol Engn, Inst Appl Chem, Beijing 100871, Peoples R China
3.Gen Hosp Chinese PLA, Med Expt & Anal Ctr, Beijing 100853, Peoples R China

Recommended Citation:
Liu, Yi,Fu, Xiaofang,Bai, Yu,et al. Improvement of reproducibility and sensitivity of CE analysis by using the capillary coated dynamically with carboxymethyl chitosan[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2011,399(8):2821-2829.
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