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题名: Piperazines for peptide carboxyl group derivatization: effect of derivatization reagents and properties of peptides on signal enhancement in matrix-assisted laser desorption/ionization mass spectrometry
作者: Qiao, Xiaoqiang1, 2;  Sun, Liangliang1, 2;  Chen, Lingfan1, 2;  Zhou, Yuan1, 2;  Yang, Kaiguang1;  Liang, Zhen1;  Zhang, Lihua1;  Zhang, Yukui1
刊名: RAPID COMMUNICATIONS IN MASS SPECTROMETRY
发表日期: 2011-03-15
DOI: 10.1002/rcm.4903
卷: 25, 期:5, 页:639-646
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences ;  Technology
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical ;  Spectroscopy
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry ;  Spectroscopy
英文摘要: Piperazine-based derivatives, including 1-(2-pyridyl) piperazine (2-PP), 1-(2-pyrimidyl) piperazine (2-PMP), 1-(4-pyridyl) piperazine (4-PP), and 1-(1-methyl-4-piperidinyl) piperazine (M-PP), were used for the derivatization of carboxyl groups on peptides with 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and 1-hydroxy-7-azabenzotriazole (HOAt) as coupling reagents, and trifluoroacetic acid (TFA) as activator. Taking synthetic peptides RVYVHPI (RI-7) and APGDRIYVHPF (AF-11) as samples, the yields of derivatized peptides by 2-PP, 2-PMP and 4-PP were higher than 94%. The effect of piperazine derivatives on the signals of tryptic digests of a-transferrin and bovine serum albumin (BSA) was investigated, and it was found that peptides derivatized by 2-PP and 2-PMP exhibited obviously improved ionization efficiency. Furthermore, comparison of identified peptides before and after derivatization showed that peptides with low molecular weight (MW) and high pI value were preferably detected after derivatization. In addition, after derivatization with 2-PP and 2-PMP, protein myelin basic protein S, 20 kDa protein, and histone H were confidently identified from the tryptic digests of two fractions of rat brain protein separated by reversed-phase high-performance liquid chromatography (HPLC), indicating the potential application of 2-PP and 2-PMP for the highly sensitive determination of peptides in comprehensive proteome analysis. Copyright (C) 2011 John Wiley & Sons, Ltd.
关键词[WOS]: ELECTROSPRAY-IONIZATION EFFICIENCY ;  AMINO-GROUPS ;  MALDI ;  PHOSPHOPEPTIDES ;  SENSITIVITY ;  PROTEOMICS ;  PROTEIN ;  MS
语种: 英语
WOS记录号: WOS:000287594900010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142655
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Qiao, Xiaoqiang,Sun, Liangliang,Chen, Lingfan,et al. Piperazines for peptide carboxyl group derivatization: effect of derivatization reagents and properties of peptides on signal enhancement in matrix-assisted laser desorption/ionization mass spectrometry[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2011,25(5):639-646.
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