DICP OpenIR
Thermally responsive polymer as a sieving matrix of proteins in capillary gel electrophoresis
Yang, Chun1,2; Chang, Chunyan1; Wang, Jun1; Qu, Qishu1; Hu, Xiaoya1; Wang, Yang1
Source PublicationANALYTICAL METHODS
2011-12-01
DOI10.1039/c1ay05443k
Volume3Issue:12Pages:2717-2720
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Life Sciences & Biomedicine ; Technology
WOS SubjectChemistry, Analytical ; Food Science & Technology ; Spectroscopy
WOS Research AreaChemistry ; Food Science & Technology ; Spectroscopy
WOS KeywordAQUEOUS CHROMATOGRAPHY ; STATIONARY PHASES ; SEPARATION ; BEADS ; SYSTEM ; DNA
AbstractA thermally responsive polymer, termed as P(IPAAm-AM-BisAM), was prepared by the copolymerization of N-isopropyl acrylamide, acrylamide, and N,N'-methylenebisacrylamide. At room temperature this polymer was dissolved in water to form a low viscous solution so that it could be loaded in a capillary easily. When a high voltage was applied on the capillary, the polymer was stimulated by the Joule heat and transferred from a liquid state into a solid state, acting as a sieving matrix for the separation of proteins according to their molecular weights. The logarithm values of the molecular weights of the proteins correlated linearly with the migration times. This capillary gel electrophoresis of proteins was carried out using a conventional instrument. Using such a copolymer, we can easily realize high-speed, high-efficiency and reproducible capillary gel electrophoresis for proteins without any instrumental rebuilding or investment.
Language英语
WOS IDWOS:000297562100002
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/142665
Collection中国科学院大连化学物理研究所
Affiliation1.Yangzhou Univ, Coll Chem & Chem Engn, Yangzhou 225002, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Yang, Chun,Chang, Chunyan,Wang, Jun,et al. Thermally responsive polymer as a sieving matrix of proteins in capillary gel electrophoresis[J]. ANALYTICAL METHODS,2011,3(12):2717-2720.
APA Yang, Chun,Chang, Chunyan,Wang, Jun,Qu, Qishu,Hu, Xiaoya,&Wang, Yang.(2011).Thermally responsive polymer as a sieving matrix of proteins in capillary gel electrophoresis.ANALYTICAL METHODS,3(12),2717-2720.
MLA Yang, Chun,et al."Thermally responsive polymer as a sieving matrix of proteins in capillary gel electrophoresis".ANALYTICAL METHODS 3.12(2011):2717-2720.
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