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Thermally responsive polymer as a sieving matrix of proteins in capillary gel electrophoresis
Yang, Chun1,2; Chang, Chunyan1; Wang, Jun1; Qu, Qishu1; Hu, Xiaoya1; Wang, Yang1
刊名ANALYTICAL METHODS
2011-12-01
DOI10.1039/c1ay05443k
3期:12页:2717-2720
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine ; Technology
类目[WOS]Chemistry, Analytical ; Food Science & Technology ; Spectroscopy
研究领域[WOS]Chemistry ; Food Science & Technology ; Spectroscopy
关键词[WOS]AQUEOUS CHROMATOGRAPHY ; STATIONARY PHASES ; SEPARATION ; BEADS ; SYSTEM ; DNA
英文摘要A thermally responsive polymer, termed as P(IPAAm-AM-BisAM), was prepared by the copolymerization of N-isopropyl acrylamide, acrylamide, and N,N'-methylenebisacrylamide. At room temperature this polymer was dissolved in water to form a low viscous solution so that it could be loaded in a capillary easily. When a high voltage was applied on the capillary, the polymer was stimulated by the Joule heat and transferred from a liquid state into a solid state, acting as a sieving matrix for the separation of proteins according to their molecular weights. The logarithm values of the molecular weights of the proteins correlated linearly with the migration times. This capillary gel electrophoresis of proteins was carried out using a conventional instrument. Using such a copolymer, we can easily realize high-speed, high-efficiency and reproducible capillary gel electrophoresis for proteins without any instrumental rebuilding or investment.
语种英语
WOS记录号WOS:000297562100002
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/142665
专题中国科学院大连化学物理研究所
作者单位1.Yangzhou Univ, Coll Chem & Chem Engn, Yangzhou 225002, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
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GB/T 7714
Yang, Chun,Chang, Chunyan,Wang, Jun,et al. Thermally responsive polymer as a sieving matrix of proteins in capillary gel electrophoresis[J]. ANALYTICAL METHODS,2011,3(12):2717-2720.
APA Yang, Chun,Chang, Chunyan,Wang, Jun,Qu, Qishu,Hu, Xiaoya,&Wang, Yang.(2011).Thermally responsive polymer as a sieving matrix of proteins in capillary gel electrophoresis.ANALYTICAL METHODS,3(12),2717-2720.
MLA Yang, Chun,et al."Thermally responsive polymer as a sieving matrix of proteins in capillary gel electrophoresis".ANALYTICAL METHODS 3.12(2011):2717-2720.
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