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题名: Thermally responsive polymer as a sieving matrix of proteins in capillary gel electrophoresis
作者: Yang, Chun1, 2;  Chang, Chunyan1;  Wang, Jun1;  Qu, Qishu1;  Hu, Xiaoya1;  Wang, Yang1
刊名: ANALYTICAL METHODS
发表日期: 2011-12-01
DOI: 10.1039/c1ay05443k
卷: 3, 期:12, 页:2717-2720
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Life Sciences & Biomedicine ;  Technology
类目[WOS]: Chemistry, Analytical ;  Food Science & Technology ;  Spectroscopy
研究领域[WOS]: Chemistry ;  Food Science & Technology ;  Spectroscopy
英文摘要: A thermally responsive polymer, termed as P(IPAAm-AM-BisAM), was prepared by the copolymerization of N-isopropyl acrylamide, acrylamide, and N,N'-methylenebisacrylamide. At room temperature this polymer was dissolved in water to form a low viscous solution so that it could be loaded in a capillary easily. When a high voltage was applied on the capillary, the polymer was stimulated by the Joule heat and transferred from a liquid state into a solid state, acting as a sieving matrix for the separation of proteins according to their molecular weights. The logarithm values of the molecular weights of the proteins correlated linearly with the migration times. This capillary gel electrophoresis of proteins was carried out using a conventional instrument. Using such a copolymer, we can easily realize high-speed, high-efficiency and reproducible capillary gel electrophoresis for proteins without any instrumental rebuilding or investment.
关键词[WOS]: AQUEOUS CHROMATOGRAPHY ;  STATIONARY PHASES ;  SEPARATION ;  BEADS ;  SYSTEM ;  DNA
语种: 英语
WOS记录号: WOS:000297562100002
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142665
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Yangzhou Univ, Coll Chem & Chem Engn, Yangzhou 225002, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Yang, Chun,Chang, Chunyan,Wang, Jun,et al. Thermally responsive polymer as a sieving matrix of proteins in capillary gel electrophoresis[J]. ANALYTICAL METHODS,2011,3(12):2717-2720.
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