DICP OpenIR
Highly efficient proteome analysis with combination of protein pre-fractionation by preparative microscale solution isoelectric focusing and identification by mu RPLC-MS/MS with serially coupled long microcolumn
Tao, Dingyin1,2; Sun, Liangliang1,2; Zhu, Guijie1; Liang, Yu1,2; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1
关键词Protein Pre-fractionation Serially Coupled Microcolumn Shotgun Proteome Analysis Mu Rplc-ms/ms
刊名JOURNAL OF SEPARATION SCIENCE
2011
DOI10.1002/jssc.201000632
34期:1页:83-89
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]TANDEM MASS-SPECTROMETRY ; FREE-FLOW ELECTROPHORESIS ; LIQUID-PHASE SEPARATION ; PREFRACTIONATION TECHNIQUES ; MULTICOMPARTMENT ELECTROLYZER ; IMMOBILINE MEMBRANES ; GEL-ELECTROPHORESIS ; SHOTGUN PROTEOMICS ; YEAST PROTEOME ; HUMAN PLASMA
英文摘要To improve the efficiency of proteome analysis, a strategy with the combination of protein pre-fractionation by preparative microscale solution isoelectric focusing, peptide separation by mu RPLC with serially coupled long microcolumn and protein identification by ESI-MS/MS was proposed. By preparative microscale solution isoelectric focusing technique, proteins extracted from whole cell lysates of Escherichia coli were fractionated into five chambers divided by isoelectric membranes, respectively with pH range from 3.0 to 4.6, 4.6 to 5.4, 5.4 to 6.2, 6.2 to 7.0 and 7.0 to 10.0. Compared to the traditional on-gel IFF, the protein recovery could be obviously improved to over 95%. Subsequently, the enriched and fractionated proteins in each chamber were digested, and further separated by a 30-cm long serially coupled RP microcolumn. Through the detection by ESI-MS/MS, about 200 proteins were identified in each fraction, and in total 835 proteins were identified even with one-dimensional mu RPLC-MS/MS system. All these results demonstrate that by such a combination strategy, highly efficient proteome analysis could be achieved, not only due to the in-solution protein enrichment and pre-fractionation with improved protein recovery but also owing to the increased separation capacity of serially coupled long mu RPLC columns.
语种英语
WOS记录号WOS:000286452700013
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/142693
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Tao, Dingyin,Sun, Liangliang,Zhu, Guijie,et al. Highly efficient proteome analysis with combination of protein pre-fractionation by preparative microscale solution isoelectric focusing and identification by mu RPLC-MS/MS with serially coupled long microcolumn[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(1):83-89.
APA Tao, Dingyin.,Sun, Liangliang.,Zhu, Guijie.,Liang, Yu.,Liang, Zhen.,...&Zhang, Yukui.(2011).Highly efficient proteome analysis with combination of protein pre-fractionation by preparative microscale solution isoelectric focusing and identification by mu RPLC-MS/MS with serially coupled long microcolumn.JOURNAL OF SEPARATION SCIENCE,34(1),83-89.
MLA Tao, Dingyin,et al."Highly efficient proteome analysis with combination of protein pre-fractionation by preparative microscale solution isoelectric focusing and identification by mu RPLC-MS/MS with serially coupled long microcolumn".JOURNAL OF SEPARATION SCIENCE 34.1(2011):83-89.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Tao, Dingyin]的文章
[Sun, Liangliang]的文章
[Zhu, Guijie]的文章
百度学术
百度学术中相似的文章
[Tao, Dingyin]的文章
[Sun, Liangliang]的文章
[Zhu, Guijie]的文章
必应学术
必应学术中相似的文章
[Tao, Dingyin]的文章
[Sun, Liangliang]的文章
[Zhu, Guijie]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。