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题名: Fast preparation of monolithic immobilized pH gradient column by photopolymerization and photografting techniques for isoelectric focusing separation of proteins
作者: Liang, Yu1, 2;  Zhu, Guijie1, 2;  Wang, Tingting1, 2;  Zhang, Xiaodan1;  Liang, Zhen1;  Zhang, Lihua1;  Zhang, Yukui1
关键词: Isoelectric focusing separation ;  Monolithic immobilized pH gradient column ;  Photografting ;  Proteins
刊名: ELECTROPHORESIS
发表日期: 2011-10-01
DOI: 10.1002/elps.201100195
卷: 32, 期:20, 页:2911-2914
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A new method was developed to prepare monolithic immobilized pH gradient (M-IPG) columns in UV-transparent fused-silica capillaries by the 5-min photopolymerization of acrylamide and N,N'-methylenebisacrylamide, followed by the 20-min photografting of the focused ampholine-derived glycidylmethacrylate monomer on the monolithic matrix, by which the preparation time was reduced, and the stability of the formed pH gradient was improved, compared with our previous methods. Using the prepared M-IPG column, the baseline separation of proteins was achieved according to their pIs. Without carrier ampholytes added in the running buffer, the separated components could be detected with high sensitivity by UV at low wavelength.
关键词[WOS]: POROUS POLYMER MONOLITHS ;  CAPILLARY-ZONE-ELECTROPHORESIS ;  SURFACE-CHEMISTRY ;  CHROMATOGRAPHY ;  ELECTROCHROMATOGRAPHY ;  DIGESTION
语种: 英语
WOS记录号: WOS:000297424500019
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142699
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

Recommended Citation:
Liang, Yu,Zhu, Guijie,Wang, Tingting,et al. Fast preparation of monolithic immobilized pH gradient column by photopolymerization and photografting techniques for isoelectric focusing separation of proteins[J]. ELECTROPHORESIS,2011,32(20):2911-2914.
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