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题名: Bioanalysis of Cepharanthine by LC-ESI-MS-MS and Its Application to Pharmacokinetic Studies
作者: Dong, Rui-Hua1;  Fang, Zhong-Ze2, 3;  Gao, Hong-Zhi1;  Hao, Guang-Tao1;  Liu, Guang1;  Shan, Ting-Ting1;  Liu, Ze-Yuan1
关键词: Column liquid chromatography-tandem mass spectrometry Pharmacokinetic study ;  Cepharanthine
刊名: CHROMATOGRAPHIA
发表日期: 2011
DOI: 10.1007/s10337-010-1823-3
卷: 73, 期:1-2, 页:75-81
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A sensitive liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS-MS) method was developed and validated for the quantification of cepharanthine (CEP) in beagle dog plasma. The chromatographic separation was performed on an Agilent-C18 column and the mobile phase was composed of methanol:water with 10 mM ammonium acetate (20:80, v/v). Detection was operated in the positive ion mode and the tandem mass spectrometer was tuned in the multiple reactions monitoring mode (MRM) to monitor m/z transitions 607 -> 365 for CEP and 285 -> 193 for the internal standard (IS) diazepam. This method exhibited a linear range of 5-2,500 ng mL(-1). The precision (RSD%) and accuracy (RME%) of the assay were < 8.7 and 2.4%, respectively. The limit of quantification was 5 ng mL(-1) and no significant matrix effect was observed. The validated method has been successfully applied to pharmacokinetic study of CEP in beagle dog.
关键词[WOS]: DRUG-METABOLISM ;  DISCOVERY ;  CELLS
语种: 英语
WOS记录号: WOS:000286670500011
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142716
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Acad Mil Med Sci, Affiliated Hosp, Dept Clin Pharmacol, Beijing 100071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Dong, Rui-Hua,Fang, Zhong-Ze,Gao, Hong-Zhi,et al. Bioanalysis of Cepharanthine by LC-ESI-MS-MS and Its Application to Pharmacokinetic Studies[J]. CHROMATOGRAPHIA,2011,73(1-2):75-81.
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