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Bioanalysis of Cepharanthine by LC-ESI-MS-MS and Its Application to Pharmacokinetic Studies
Dong, Rui-Hua1; Fang, Zhong-Ze2,3; Gao, Hong-Zhi1; Hao, Guang-Tao1; Liu, Guang1; Shan, Ting-Ting1; Liu, Ze-Yuan1
关键词Column Liquid Chromatography-tandem Mass Spectrometry Pharmacokinetic Study Cepharanthine
刊名CHROMATOGRAPHIA
2011
DOI10.1007/s10337-010-1823-3
73期:1-2页:75-81
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]DRUG-METABOLISM ; DISCOVERY ; CELLS
英文摘要A sensitive liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS-MS) method was developed and validated for the quantification of cepharanthine (CEP) in beagle dog plasma. The chromatographic separation was performed on an Agilent-C18 column and the mobile phase was composed of methanol:water with 10 mM ammonium acetate (20:80, v/v). Detection was operated in the positive ion mode and the tandem mass spectrometer was tuned in the multiple reactions monitoring mode (MRM) to monitor m/z transitions 607 -> 365 for CEP and 285 -> 193 for the internal standard (IS) diazepam. This method exhibited a linear range of 5-2,500 ng mL(-1). The precision (RSD%) and accuracy (RME%) of the assay were < 8.7 and 2.4%, respectively. The limit of quantification was 5 ng mL(-1) and no significant matrix effect was observed. The validated method has been successfully applied to pharmacokinetic study of CEP in beagle dog.
语种英语
WOS记录号WOS:000286670500011
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/142716
专题中国科学院大连化学物理研究所
作者单位1.Acad Mil Med Sci, Affiliated Hosp, Dept Clin Pharmacol, Beijing 100071, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
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GB/T 7714
Dong, Rui-Hua,Fang, Zhong-Ze,Gao, Hong-Zhi,et al. Bioanalysis of Cepharanthine by LC-ESI-MS-MS and Its Application to Pharmacokinetic Studies[J]. CHROMATOGRAPHIA,2011,73(1-2):75-81.
APA Dong, Rui-Hua.,Fang, Zhong-Ze.,Gao, Hong-Zhi.,Hao, Guang-Tao.,Liu, Guang.,...&Liu, Ze-Yuan.(2011).Bioanalysis of Cepharanthine by LC-ESI-MS-MS and Its Application to Pharmacokinetic Studies.CHROMATOGRAPHIA,73(1-2),75-81.
MLA Dong, Rui-Hua,et al."Bioanalysis of Cepharanthine by LC-ESI-MS-MS and Its Application to Pharmacokinetic Studies".CHROMATOGRAPHIA 73.1-2(2011):75-81.
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