DICP OpenIR
Cellular Localization of Debromohymenialdisine and Hymenialdisine in the Marine Sponge Axinella sp Using a Newly Developed Cell Purification Protocol
Song, Yue-Fan3,4; Qu, Yi3,4; Cao, Xu-Peng3; Zhang, Wei1,2,3
KeywordPorifera Marine Sponge Cell Purification Debromohymenialdisine Hymenialdisine Localization
Source PublicationMARINE BIOTECHNOLOGY
2011-10-01
DOI10.1007/s10126-010-9347-2
Volume13Issue:5Pages:868-882
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
WOS SubjectBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS KeywordFRESH-WATER SPONGE ; EPHYDATIA-FLUVIATILIS ; HYMENIACIDON-PERLEVE ; POLYPOIDES SCHMIDT ; NATURAL-PRODUCTS ; DYSIDEA-AVARA ; STEM-CELLS ; DEMOSPONGIAE ; ORIGIN ; METABOLITES
AbstractSponges (Porifera), as the best known source of bioactive marine natural products in metazoans, play a significant role in marine drug discovery and development. As sessile filter-feeding animals, a considerable portion of the sponge biomass can be made of endosymbiotic and associated microorganisms. Understanding the cellular origin of targeted bioactive compounds from sponges is therefore important not only for providing chemotaxonomic information but also for defining the bioactive production strategy in terms of sponge aquaculture, cell culture, or fermentation of associated bacteria. The two alkaloids debromohymenialdisine (DBH) and hymenialdisine (HD), which are cyclin-dependent kinase inhibitors with pharmacological activities for treating osteoarthritis and Alzheimer's disease, have been isolated from the sponge Axinella sp. In this study, the cellular localization of these two alkaloids was determined through the quantification of these alkaloids in different cell fractions by high-performance liquid chromatography (HPLC). First, using a differential centrifugation method, the dissociated cells were separated into different groups according to their sizes. The two bioactive alkaloids were mainly found in sponge cells obtained from low-speed centrifugation. Further cell purifications were accomplished by a newly developed multi-step protocol. Four enriched cell fractions (C1, C2, C3, and C4) were obtained and subjected to light and transmission electron microscopy, cytochemical staining, and HPLC quantification. Compared to the low concentrations in other cell fractions, DBH and HD accounted for 10.9% and 6.1%, respectively, of dry weight in the C1 fraction. Using the morphological characteristics and cytochemical staining results, cells in the C1 fraction were speculated to be spherulous cells. This result shows that DBH and HD in Axinella sp. are located in sponge cells and mostly stored in spherulous cells.
Language英语
WOS IDWOS:000293970600006
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/142718
Collection中国科学院大连化学物理研究所
Affiliation1.Flinders Univ S Australia, Sch Med, Flinders Ctr Marine Bioproc & Bioprod, Adelaide, SA 5042, Australia
2.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Adelaide, SA 5042, Australia
3.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Liaoning, Peoples R China
4.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Song, Yue-Fan,Qu, Yi,Cao, Xu-Peng,et al. Cellular Localization of Debromohymenialdisine and Hymenialdisine in the Marine Sponge Axinella sp Using a Newly Developed Cell Purification Protocol[J]. MARINE BIOTECHNOLOGY,2011,13(5):868-882.
APA Song, Yue-Fan,Qu, Yi,Cao, Xu-Peng,&Zhang, Wei.(2011).Cellular Localization of Debromohymenialdisine and Hymenialdisine in the Marine Sponge Axinella sp Using a Newly Developed Cell Purification Protocol.MARINE BIOTECHNOLOGY,13(5),868-882.
MLA Song, Yue-Fan,et al."Cellular Localization of Debromohymenialdisine and Hymenialdisine in the Marine Sponge Axinella sp Using a Newly Developed Cell Purification Protocol".MARINE BIOTECHNOLOGY 13.5(2011):868-882.
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