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题名: Ultra-high capacity liquid chromatography chip/quadrupole time-of-flight mass spectrometry for pharmaceutical analysis
作者: Zhao, Chunxia1;  Wu, Zeming1;  Xue, Gang2;  Wang, Jian2;  Zhao, Yining2;  Xu, Zhixiu3;  Lin, Dayin3;  Herbert, Godel3;  Chang, Yuwei1;  Cai, Kaiyang1;  Xu, Guowang1
关键词: Ultra-high capacity chip ;  Pharmaceutical analysis ;  Liquid chromatography ;  Mass spectrometry ;  HPLC-Chip
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2011-06-10
DOI: 10.1016/j.chroma.2011.04.020
卷: 1218, 期:23, 页:3669-3674
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Nanoflow liquid chromatography/mass spectrometry (nano-LC/MS) has attracted increasing interest ill virtue of high sensitivity, low sample consumption, and minimal matrix effect. In this work a HPLC-Chip/quadrupole time-of-flight (Q-TOF) MS device with a new ultra-high capacity small molecule chip (UHC-Chip) which features a 500 nL enrichment column and a 150 mm x 75 mu m analytical column, was evaluated with a drug mixture covering a wide range of polarities. Excellent chromatographic precision with 0.1-0.5% RSD for retention time and 1.7-9.0% RSD for peak area, low limit of detection, good chip-to-chip reproducibility and linearity were obtained by using this UHC-Chip. Compared with the standard HPLC-Chip with 40 nL trapping column, the UHC-Chip showed higher enrichment capability and hence gave a higher response in signal detection. Additionally, 4-30 times increase in sensitivity was obtained compared with conventional LC/MS, which indicated that UHC-Chip/MS was a valuable tool for the quantitative analysis of low level impurities and degradation products in pharmaceuticals. Moreover, satisfactory results obtained from trace drug analysis of serum samples further proved its practicality and potential for use in drug testing and development. (C) 2011 Elsevier B.V. All rights reserved.
关键词[WOS]: INTEGRATED MICROFLUIDIC DEVICE ;  DRUG-DELIVERY ;  HPLC-CHIP ;  IDENTIFICATION ;  PROTEOMICS ;  PARTICLES ;  LC/MS
语种: 英语
WOS记录号: WOS:000291521200013
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142721
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Pfizer Inc, Pfizer Global R&D, Groton, CT 06340 USA
3.Agilent Technol, Waldbronn, Germany

Recommended Citation:
Zhao, Chunxia,Wu, Zeming,Xue, Gang,et al. Ultra-high capacity liquid chromatography chip/quadrupole time-of-flight mass spectrometry for pharmaceutical analysis[J]. JOURNAL OF CHROMATOGRAPHY A,2011,1218(23):3669-3674.
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