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Matrix of 3,4-Diaminobenzophenone for Analysis of Phospholipids by Matrix-Assisted Laser Desorption/Ionization-Time of Flight-Mass Spectrometry
Miao Nan1; Zhang Yu1; Zhang Jian-Ye1,2; Zou Han-Fa1
关键词Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry Matrix Phospholipids 3 4-diaminobenzophenone
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2011-05-01
DOI10.3724/SP.J.1096.2011.00605
39期:5页:605-610
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]MALDI-TOF-MS ; LIPID ANALYSIS ; DESORPTION ; CHROMATOGRAPHY ; SEPARATION ; MIXTURES ; PEPTIDE ; SAMPLES
英文摘要The matrix of 3, 4-diaminobenzophenone(DABP) was applied in the analysis of phospholipids by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The optimal composition of DABP matrix solution with methanol-water-hydrochloric acid (80 20 3, V/V) was used in the analysis of different phospholipids species by comparing with the conventional matrices such as 2,4,6-trihydroxyacetophenone(THAP), 2,5-dihydroxy-benzoic acid (DHB) and alpha-cynao-4-hydroxycinnamic acid (CHCA), 3, 4-diamino-benzophenone ( DABP) can effectively suppress alkali metal ion adducts, thus showing single peak of phospholipids with low laser energy and strong peak strength. 500 fmol phosphatidylcholines(PCs) can be detected with high signal to noise ratio. With DABP as a matrix for the analysis of egg yolk and soybean extracts, intact phospholipids ion peaks can be readily produced with clean spectrum background and high resolution. In addition, the sample preparation method is simple and time-saving.
语种英语
WOS记录号WOS:000293202300001
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/142723
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Zhengzhou Univ, Dept Chem, Zhengzhou 450052, Peoples R China
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Miao Nan,Zhang Yu,Zhang Jian-Ye,et al. Matrix of 3,4-Diaminobenzophenone for Analysis of Phospholipids by Matrix-Assisted Laser Desorption/Ionization-Time of Flight-Mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2011,39(5):605-610.
APA Miao Nan,Zhang Yu,Zhang Jian-Ye,&Zou Han-Fa.(2011).Matrix of 3,4-Diaminobenzophenone for Analysis of Phospholipids by Matrix-Assisted Laser Desorption/Ionization-Time of Flight-Mass Spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,39(5),605-610.
MLA Miao Nan,et al."Matrix of 3,4-Diaminobenzophenone for Analysis of Phospholipids by Matrix-Assisted Laser Desorption/Ionization-Time of Flight-Mass Spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 39.5(2011):605-610.
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