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Determination of propofol UDP-glucuronosyltransferase (UGT) activities in hepatic microsomes from different species by UFLC-ESI-MS
Liang, Si-Cheng1; Ge, Guang-Bo1; Liu, Hui-Xin1; Shang, Hai-Tao2; Wei, Hong2; Fang, Zhong-Ze1; Zhu, Liang-Liang1; Mao, Yu-Xi1; Yang, Ling1
关键词Ultra-fast Liquid Chromatography Propofol O-glucuronide Hepatic Microsomes Interspecies Difference Human Individual Difference
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
2011-01-05
DOI10.1016/j.jpba.2010.07.049
54期:1页:236-241
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine
类目[WOS]Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]LIQUID-CHROMATOGRAPHIC ASSAY ; DRUG GLUCURONIDATION ; LIVER-MICROSOMES ; METABOLITES ; URINE ; PHARMACOKINETICS ; PREDICTION
英文摘要Propofol O-glucuronidation has been used as probe reaction to phenotype UGT1A9 activity in human liver, thus a sensitive and specific method for determination of propofol O-glucuronide (PG) is urgently desirable. In the current study, a new LC-ESI-MS method for determination of PG in hepatic microsomes from human (HLM), monkey (CyLM), dog (DLM), minipig (PLM), rat (RLM) and mouse (MLM) was developed and validated using 4-methylumbelliferyl-beta-D-glucuronide as an internal standard (IS). PG and IS was separated by a Shim-pack XR-ODS column (100 mm x 2.0 mm, 2.2 mu m, Shimadzu) under gradient conditions with the mobile phase of acetonitrile and water containing 0.2% acetic acid (v/v). The mass spectrometric detection was performed under selected ion monitoring (SIM) for PG at m/z 353 and IS at m/z 351. The assay exhibited linearity over the range 0.05-30 mu M for PG with the correlation coefficient of 0.9995. The intra- and inter-day precision was less than 7.2%, with accuracy in the range 93.8-107.5%. The developed method was successfully used for characterizing interspecies and human individual differences in the O-glucuronidation activity towards propofol, as well as investigating inhibitory effects of androsterone and phenylbutazone on propofol O-glucuronidation in HLM. (c) 2010 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000282863300037
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/142726
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Pharmaceut Resource Discovery, Dalian, Peoples R China
2.Third Mil Med Univ, Chongqing, Peoples R China
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GB/T 7714
Liang, Si-Cheng,Ge, Guang-Bo,Liu, Hui-Xin,et al. Determination of propofol UDP-glucuronosyltransferase (UGT) activities in hepatic microsomes from different species by UFLC-ESI-MS[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2011,54(1):236-241.
APA Liang, Si-Cheng.,Ge, Guang-Bo.,Liu, Hui-Xin.,Shang, Hai-Tao.,Wei, Hong.,...&Yang, Ling.(2011).Determination of propofol UDP-glucuronosyltransferase (UGT) activities in hepatic microsomes from different species by UFLC-ESI-MS.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,54(1),236-241.
MLA Liang, Si-Cheng,et al."Determination of propofol UDP-glucuronosyltransferase (UGT) activities in hepatic microsomes from different species by UFLC-ESI-MS".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 54.1(2011):236-241.
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