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Regioselective Galactosylation of Floxuridine Catalyzed by beta-Galactosidase from Bovine Liver in Co-solvent Systems
Ye Min2; Liu Qiuping2; Li Ning1,3; Zong Minhua1
KeywordBeta-galactosidase Glycosylation Regioselectivity Floxuridine Organic Solvent
Source PublicationCHINESE JOURNAL OF CATALYSIS
2011-06-01
DOI10.3724/SP.J.1088.2011.01150
Volume32Issue:6Pages:1063-1068
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
WOS SubjectChemistry, Applied ; Chemistry, Physical ; Engineering, Chemical
WOS Research AreaChemistry ; Engineering
WOS KeywordDISACCHARIDE NUCLEOSIDES ; N-ACETYLLACTOSAMINE ; PYROCOCCUS-FURIOSUS ; ORGANIC-SOLVENTS ; GLUCOSIDASE ; MEDIA ; 5-FLUOROURIDINE ; STABILITY
AbstractThe influence of organic solvents and their contents on the regioselective galactosylation of floxuridine catalyzed by if-galactosidase from bovine liver was investigated in co-solvent systems. It was revealed that the enzyme was unstable and inactivated substantially in organic solvent (10%, v/v)-containing systems, and the detrimental impacts of organic solvents on the enzyme could be relieved remarkably by decreasing their contents. Nevertheless, the addition of organic solvents exerted no influence on the regioselectivity of the enzymatic glycosylation, which retained >99%. The catalytic performance of beta-galactosidase from bovine liver correlated well with the dielectric constants of organic solvents in co-solvent systems, among which the enzyme displayed the highest activity in dimethyl sulfoxide (DMSO)-containing system. The yield of desired product reached 71% when the DMSO content was less than 5% (v/v) in the medium.
Language英语
WOS IDWOS:000292429000026
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/142735
Collection中国科学院大连化学物理研究所
Affiliation1.S China Univ Technol, Coll Light Ind & Food Sci, State Key Lab Pulp & Paper Engn, Guangzhou 510640, Guangdong, Peoples R China
2.S China Univ Technol, Sch Biosci & Bioengn, Guangzhou 510006, Guangdong, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Liaoning, Peoples R China
Recommended Citation
GB/T 7714
Ye Min,Liu Qiuping,Li Ning,et al. Regioselective Galactosylation of Floxuridine Catalyzed by beta-Galactosidase from Bovine Liver in Co-solvent Systems[J]. CHINESE JOURNAL OF CATALYSIS,2011,32(6):1063-1068.
APA Ye Min,Liu Qiuping,Li Ning,&Zong Minhua.(2011).Regioselective Galactosylation of Floxuridine Catalyzed by beta-Galactosidase from Bovine Liver in Co-solvent Systems.CHINESE JOURNAL OF CATALYSIS,32(6),1063-1068.
MLA Ye Min,et al."Regioselective Galactosylation of Floxuridine Catalyzed by beta-Galactosidase from Bovine Liver in Co-solvent Systems".CHINESE JOURNAL OF CATALYSIS 32.6(2011):1063-1068.
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