中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Regioselective Galactosylation of Floxuridine Catalyzed by beta-Galactosidase from Bovine Liver in Co-solvent Systems
作者: Ye Min2;  Liu Qiuping2;  Li Ning1, 3;  Zong Minhua1
关键词: beta-galactosidase ;  glycosylation ;  regioselectivity ;  floxuridine ;  organic solvent
刊名: CHINESE JOURNAL OF CATALYSIS
发表日期: 2011-06-01
DOI: 10.3724/SP.J.1088.2011.01150
卷: 32, 期:6, 页:1063-1068
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Applied ;  Chemistry, Physical ;  Engineering, Chemical
研究领域[WOS]: Chemistry ;  Engineering
英文摘要: The influence of organic solvents and their contents on the regioselective galactosylation of floxuridine catalyzed by if-galactosidase from bovine liver was investigated in co-solvent systems. It was revealed that the enzyme was unstable and inactivated substantially in organic solvent (10%, v/v)-containing systems, and the detrimental impacts of organic solvents on the enzyme could be relieved remarkably by decreasing their contents. Nevertheless, the addition of organic solvents exerted no influence on the regioselectivity of the enzymatic glycosylation, which retained >99%. The catalytic performance of beta-galactosidase from bovine liver correlated well with the dielectric constants of organic solvents in co-solvent systems, among which the enzyme displayed the highest activity in dimethyl sulfoxide (DMSO)-containing system. The yield of desired product reached 71% when the DMSO content was less than 5% (v/v) in the medium.
关键词[WOS]: DISACCHARIDE NUCLEOSIDES ;  N-ACETYLLACTOSAMINE ;  PYROCOCCUS-FURIOSUS ;  ORGANIC-SOLVENTS ;  GLUCOSIDASE ;  MEDIA ;  5-FLUOROURIDINE ;  STABILITY
语种: 英语
WOS记录号: WOS:000292429000026
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142735
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.S China Univ Technol, Coll Light Ind & Food Sci, State Key Lab Pulp & Paper Engn, Guangzhou 510640, Guangdong, Peoples R China
2.S China Univ Technol, Sch Biosci & Bioengn, Guangzhou 510006, Guangdong, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Liaoning, Peoples R China

Recommended Citation:
Ye Min,Liu Qiuping,Li Ning,et al. Regioselective Galactosylation of Floxuridine Catalyzed by beta-Galactosidase from Bovine Liver in Co-solvent Systems[J]. CHINESE JOURNAL OF CATALYSIS,2011,32(6):1063-1068.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Ye Min]'s Articles
 [Liu Qiuping]'s Articles
 [Li Ning]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Ye Min]‘s Articles
 [Liu Qiuping]‘s Articles
 [Li Ning]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace