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Regioselective Galactosylation of Floxuridine Catalyzed by beta-Galactosidase from Bovine Liver in Co-solvent Systems
Ye Min2; Liu Qiuping2; Li Ning1,3; Zong Minhua1
关键词Beta-galactosidase Glycosylation Regioselectivity Floxuridine Organic Solvent
刊名CHINESE JOURNAL OF CATALYSIS
2011-06-01
DOI10.3724/SP.J.1088.2011.01150
32期:6页:1063-1068
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences ; Technology
类目[WOS]Chemistry, Applied ; Chemistry, Physical ; Engineering, Chemical
研究领域[WOS]Chemistry ; Engineering
关键词[WOS]DISACCHARIDE NUCLEOSIDES ; N-ACETYLLACTOSAMINE ; PYROCOCCUS-FURIOSUS ; ORGANIC-SOLVENTS ; GLUCOSIDASE ; MEDIA ; 5-FLUOROURIDINE ; STABILITY
英文摘要The influence of organic solvents and their contents on the regioselective galactosylation of floxuridine catalyzed by if-galactosidase from bovine liver was investigated in co-solvent systems. It was revealed that the enzyme was unstable and inactivated substantially in organic solvent (10%, v/v)-containing systems, and the detrimental impacts of organic solvents on the enzyme could be relieved remarkably by decreasing their contents. Nevertheless, the addition of organic solvents exerted no influence on the regioselectivity of the enzymatic glycosylation, which retained >99%. The catalytic performance of beta-galactosidase from bovine liver correlated well with the dielectric constants of organic solvents in co-solvent systems, among which the enzyme displayed the highest activity in dimethyl sulfoxide (DMSO)-containing system. The yield of desired product reached 71% when the DMSO content was less than 5% (v/v) in the medium.
语种英语
WOS记录号WOS:000292429000026
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/142735
专题中国科学院大连化学物理研究所
作者单位1.S China Univ Technol, Coll Light Ind & Food Sci, State Key Lab Pulp & Paper Engn, Guangzhou 510640, Guangdong, Peoples R China
2.S China Univ Technol, Sch Biosci & Bioengn, Guangzhou 510006, Guangdong, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Liaoning, Peoples R China
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GB/T 7714
Ye Min,Liu Qiuping,Li Ning,et al. Regioselective Galactosylation of Floxuridine Catalyzed by beta-Galactosidase from Bovine Liver in Co-solvent Systems[J]. CHINESE JOURNAL OF CATALYSIS,2011,32(6):1063-1068.
APA Ye Min,Liu Qiuping,Li Ning,&Zong Minhua.(2011).Regioselective Galactosylation of Floxuridine Catalyzed by beta-Galactosidase from Bovine Liver in Co-solvent Systems.CHINESE JOURNAL OF CATALYSIS,32(6),1063-1068.
MLA Ye Min,et al."Regioselective Galactosylation of Floxuridine Catalyzed by beta-Galactosidase from Bovine Liver in Co-solvent Systems".CHINESE JOURNAL OF CATALYSIS 32.6(2011):1063-1068.
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