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题名: The use of injectable sonication-induced silk hydrogel for VEGF(165) and BMP-2 delivery for elevation of the maxillary sinus floor
作者: Zhang, Wenjie2;  Wang, Xiuli3, 4;  Wang, Shaoyi5;  Zhao, Jun5;  Xu, Lianyi2;  Zhu, Chao5;  Zeng, Deliang2;  Chen, Jake6;  Zhang, Zhiyuan5;  Kaplan, David L.4;  Jiang, Xinquan1, 2
关键词: Silk fibron ;  Hydrogels ;  Sonication ;  VEGF ;  BMP-2 ;  Maxillary sinus augmentation
刊名: BIOMATERIALS
发表日期: 2011-12-01
DOI: 10.1016/j.biomaterials.2011.08.047
卷: 32, 期:35, 页:9415-9424
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Technology
类目[WOS]: Engineering, Biomedical ;  Materials Science, Biomaterials
研究领域[WOS]: Engineering ;  Materials Science
英文摘要: Sonication-induced silk hydrogels were previously prepared as an injectable bone replacement biomaterial, with a need to improve osteogenic features. Vascular endothelial growth factor (VEGF(165)) and bone morphogenic protein-2 (BMP-2) are key regulators of angiogenesis and osteogenesis, respectively, during bone regeneration. Therefore, the present study aimed at evaluating in situ forming silk hydrogels as a vehicle to encapsulate dual factors for rabbit maxillary sinus floor augmentation. Sonication-induced silk hydrogels were prepared in vitro and the slow release of VEGF(165) and BMP-2 from these silk gels was evaluated by ELISA. For in vivo studies for each time point (4 and 12 weeks), 24 sinus floors elevation surgeries were made bilaterally in 12 rabbits for the following four treatment groups: silk gel (group Silk gel), silk gel/VEGF(165) (group VEGF), silk gel/BMP-2 (group BMP-2), silk gel/VEGF(165)/BMP-2 (group V + B) (n = 6 per group). Sequential florescent labeling and radiographic observations were used to record new bone formation and mineralization, along with histological and histomorphometric analysis. At week 4, VEGF(165) promoted more tissue infiltration into the gel and accelerated the degradation of the gel material. At this time point, the bone area in group V + B was significantly larger than those in the other three groups. At week 12, elevated sinus floor heights of groups BMP-2 and V + B were larger than those of the Silk gel and VEGF groups, and the V + B group had the largest new bone area among all groups. In addition, a larger blood vessel area formed in the remaining gel areas in groups VEGF and V + B. In conclusion, VEGF(165) and BMP-2 released from injectable and biodegradable silk gels promoted angiogenesis and new bone formation, with the two factors demonstrating an additive effect on bone regeneration. These results indicate that silk hydrogels can be used as an injectable vehicle to deliver multiple growth factors in a minimally invasive approach to regenerate irregular bony cavities. (C) 2011 Elsevier Ltd. All rights reserved.
关键词[WOS]: BONE MORPHOGENETIC PROTEIN-2 ;  TISSUE-ENGINEERED BONE ;  IN-VIVO ;  FIBROIN SCAFFOLDS ;  AUGMENTATION ;  REPAIR ;  VEGF ;  BIOMATERIALS ;  ANGIOGENESIS ;  REGENERATION
语种: 英语
WOS记录号: WOS:000296684200027
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142737
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Shanghai Jiao Tong Univ, Peoples Hosp 9, Sch Med, Dept Prosthodont, Shanghai 200011, Peoples R China
2.Shanghai Jiao Tong Univ, Oral Bioengn & Regenerat Med Lab, Shanghai Res Inst Stomatol, Sch Med,Shanghai Key Lab Stomatol,Peoples Hosp 9, Shanghai 200011, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem & Phys, Dalian 116023, Peoples R China
4.Tufts Univ, Dept Biomed Engn, Sch Engn, Medford, MA 02155 USA
5.Shanghai Jiao Tong Univ, Sch Med, Dept Oral & Maxillofacial Surg, Peoples Hosp 9, Shanghai 200011, Peoples R China
6.Tufts Univ, Sch Dent Med, Div Oral Biol, Boston, MA 02111 USA

Recommended Citation:
Zhang, Wenjie,Wang, Xiuli,Wang, Shaoyi,et al. The use of injectable sonication-induced silk hydrogel for VEGF(165) and BMP-2 delivery for elevation of the maxillary sinus floor[J]. BIOMATERIALS,2011,32(35):9415-9424.
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