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Synthesis and application of a macroporous boronate affinity monolithic column using a metal-organic gel as a porogenic template for the specific capture of glycoproteins
Yang, Fan1; Lin, Zian2; He, Xiwen1; Chen, Langxing1; Zhang, Yukui1,3
关键词Metal-organic Gels Boronate Affinity Chromatography Glycoproteins Macroporous Monolithic Column
刊名JOURNAL OF CHROMATOGRAPHY A
2011-12-23
DOI10.1016/j.chroma.2011.10.049
1218期:51页:9194-9201
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]SOLID-PHASE EXTRACTION ; MAGNETIC NANOPARTICLES ; POLYMER MONOLITHS ; GLYCOPEPTIDES ; CHROMATOGRAPHY ; ENRICHMENT
英文摘要A macroporous boronate affinity monolithic column was prepared and applied to specifically capture glycoproteins using metal-organic gels (MOGs) as a porogenic template. This newly explored application of MOGs has proven to be a more convenient method for the formation of macropores in contrast to traditional porogenic methods. The poly (3-acrylamidophenylboronic acid-co-ethylene dimethacrylate) monolithic columns were synthesized in stainless columns by in situ polymerization. To fabricate the macroporous formation with a uniformed open-channel network, the preparation conditions, such as reaction temperature, the concentration of the MOGs and the ratio of monomers were systematically investigated. The prepared macroporous monoliths were characterized by scanning electron microscope (SEM) and mercury intrusion porosimetry. Furthermore, horseradish peroxidase (HRP) and transferrin (TF) were chosen as test glycoproteins, and the chromatographic analysis demonstrated that the macroporous boronate affinity monoliths exhibited a higher selectivity and better dynamic binding capacity toward glycoproteins compared with non-glycoproteins. The resulted affinity monolithic column was successfully employed to specifically capture TF from a bovine serum sample. (C) 2011 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000298530000010
引用统计
被引频次:66[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/142738
专题中国科学院大连化学物理研究所
作者单位1.Nankai Univ, Coll Chem, Tianjin 300071, Peoples R China
2.Fuzhou Univ, Dept Chem, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Minist Educ,Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China
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Yang, Fan,Lin, Zian,He, Xiwen,et al. Synthesis and application of a macroporous boronate affinity monolithic column using a metal-organic gel as a porogenic template for the specific capture of glycoproteins[J]. JOURNAL OF CHROMATOGRAPHY A,2011,1218(51):9194-9201.
APA Yang, Fan,Lin, Zian,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2011).Synthesis and application of a macroporous boronate affinity monolithic column using a metal-organic gel as a porogenic template for the specific capture of glycoproteins.JOURNAL OF CHROMATOGRAPHY A,1218(51),9194-9201.
MLA Yang, Fan,et al."Synthesis and application of a macroporous boronate affinity monolithic column using a metal-organic gel as a porogenic template for the specific capture of glycoproteins".JOURNAL OF CHROMATOGRAPHY A 1218.51(2011):9194-9201.
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