中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Synthesis and application of a macroporous boronate affinity monolithic column using a metal-organic gel as a porogenic template for the specific capture of glycoproteins
作者: Yang, Fan1;  Lin, Zian2;  He, Xiwen1;  Chen, Langxing1;  Zhang, Yukui1, 3
关键词: Metal-organic gels ;  Boronate affinity chromatography ;  Glycoproteins ;  Macroporous monolithic column
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2011-12-23
DOI: 10.1016/j.chroma.2011.10.049
卷: 1218, 期:51, 页:9194-9201
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A macroporous boronate affinity monolithic column was prepared and applied to specifically capture glycoproteins using metal-organic gels (MOGs) as a porogenic template. This newly explored application of MOGs has proven to be a more convenient method for the formation of macropores in contrast to traditional porogenic methods. The poly (3-acrylamidophenylboronic acid-co-ethylene dimethacrylate) monolithic columns were synthesized in stainless columns by in situ polymerization. To fabricate the macroporous formation with a uniformed open-channel network, the preparation conditions, such as reaction temperature, the concentration of the MOGs and the ratio of monomers were systematically investigated. The prepared macroporous monoliths were characterized by scanning electron microscope (SEM) and mercury intrusion porosimetry. Furthermore, horseradish peroxidase (HRP) and transferrin (TF) were chosen as test glycoproteins, and the chromatographic analysis demonstrated that the macroporous boronate affinity monoliths exhibited a higher selectivity and better dynamic binding capacity toward glycoproteins compared with non-glycoproteins. The resulted affinity monolithic column was successfully employed to specifically capture TF from a bovine serum sample. (C) 2011 Elsevier B.V. All rights reserved.
关键词[WOS]: SOLID-PHASE EXTRACTION ;  MAGNETIC NANOPARTICLES ;  POLYMER MONOLITHS ;  GLYCOPEPTIDES ;  CHROMATOGRAPHY ;  ENRICHMENT
语种: 英语
WOS记录号: WOS:000298530000010
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142738
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Nankai Univ, Coll Chem, Tianjin 300071, Peoples R China
2.Fuzhou Univ, Dept Chem, Fujian Prov Key Lab Anal & Detect Technol Food Sa, Minist Educ,Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China

Recommended Citation:
Yang, Fan,Lin, Zian,He, Xiwen,et al. Synthesis and application of a macroporous boronate affinity monolithic column using a metal-organic gel as a porogenic template for the specific capture of glycoproteins[J]. JOURNAL OF CHROMATOGRAPHY A,2011,1218(51):9194-9201.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Yang, Fan]'s Articles
 [Lin, Zian]'s Articles
 [He, Xiwen]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Yang, Fan]‘s Articles
 [Lin, Zian]‘s Articles
 [He, Xiwen]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace