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题名: CENP-E Kinesin Interacts with SKAP Protein to Orchestrate Accurate Chromosome Segregation in Mitosis
作者: Huang, Yuejia1, 2;  Wang, Wenwen1;  Yao, Phil2;  Wang, Xiwei1, 2;  Liu, Xing1, 2;  Zhuang, Xiaoxuan1;  Yan, Feng1, 2;  Zhou, Jinhua1;  Du, Jian1;  Ward, Tarsha2;  Zou, Hanfa3;  Zhang, Jiancun1, 5;  Fang, Guowei1;  Ding, Xia4;  Dou, Zhen1;  Yao, Xuebiao1
刊名: JOURNAL OF BIOLOGICAL CHEMISTRY
发表日期: 2012-01-06
DOI: 10.1074/jbc.M111.277194
卷: 287, 期:2, 页:1500-1509
收录类别: SCI
文章类型: Article
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
英文摘要: Mitotic chromosome segregation is orchestrated by the dynamic interaction of spindle microtubules with the kinetochore. Although previous studies show that the mitotic kinesin CENP-E forms a link between attachment of the spindle microtubule to the kinetochore and the mitotic checkpoint signaling cascade, the molecular mechanism underlying dynamic kinetochore-microtubule interactions in mammalian cells remains elusive. Here, we identify a novel interaction between CENP-E and SKAP that functions synergistically in governing dynamic kinetochore-microtubule interactions. SKAP binds to the C-terminal tail of CENP-E in vitro and is essential for an accurate kinetochore-microtubule attachment in vivo. Immunoelectron microscopic analysis indicates that SKAP is a constituent of the kinetochore corona fibers of mammalian centromeres. Depletion of SKAP or CENP-E by RNA interference results in a dramatic reduction of inter-kinetochore tension, which causes chromosome mis-segregation with a prolonged delay in achieving metaphase alignment. Importantly, SKAP binds to microtubules in vitro, and this interaction is synergized by CENP-E. Based on these findings, we propose that SKAP cooperates with CENP-E to orchestrate dynamic kinetochore-microtubule interaction for faithful chromosome segregation.
关键词[WOS]: MICROTUBULE PLUS ENDS ;  MITOTIC CHECKPOINT ;  SPINDLE MICROTUBULES ;  KINETOCHORE PROTEIN ;  OUTER PLATE ;  ATTACHMENT ;  LOCALIZATION ;  MOTOR ;  ANEUPLOIDY ;  COMPLEX
语种: 英语
WOS记录号: WOS:000299170300063
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142833
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Univ Sci & Technol China, Anhui Key Lab Cellular Dynam & Chem Biol, Sch Life Sci, Hefei 230027, Peoples R China
2.Morehouse Sch Med, Dept Physiol, Atlanta, GA 30310 USA
3.Dalian Inst Chem Phys, Dalian 116023, Peoples R China
4.Beijing Univ Chinese Med, Sch Grad Studies, Beijing 100029, Peoples R China
5.Guangzhou Inst Hlth Sci, Guangzhou 510515, Guangdong, Peoples R China

Recommended Citation:
Huang, Yuejia,Wang, Wenwen,Yao, Phil,et al. CENP-E Kinesin Interacts with SKAP Protein to Orchestrate Accurate Chromosome Segregation in Mitosis[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2012,287(2):1500-1509.
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