DICP OpenIR
Multiparameter evaluation of the longevity in C-elegans under stress using an integrated microfluidic device
Wen, Hui; Shi, Weiwei; Qin, Jianhua
KeywordMicrofluidic C. Elegans Longevity Polydatin
Source PublicationBIOMEDICAL MICRODEVICES
2012-08-01
DOI10.1007/s10544-012-9652-9
Volume14Issue:4Pages:721-728
Indexed BySCI
SubtypeArticle
WOS SubjectEngineering, Biomedical ; Nanoscience & Nanotechnology
WOS Research AreaEngineering ; Science & Technology - Other Topics
AbstractCaenorhabditis elegans (C. elegans) is an excellent model organism for the study of aging and longevity. In this work, we presented a microfluidic approach for the evaluation of longevity in C. elegans under stress. The microfluidic device integrated multiple microvalves with parallel channels, which enabled the long-term culture and flexible manipulation of C. elegans in real-time. The utility of the device was demonstrated by characterizing the lifespan, mobility behavior and fluorescence expression of oxidative stress in mutant strain CL2166 simultaneously at single animal resolution. A certain dose of polydatin was found to enable the extension of mean lifespan of CL2166 for the first time, and the prolonged longevity activity was possibly mediated by the protective response to oxidative stress, indicating the promising role of polydatin involved in aging process. The device is simple to operate, easy for real-time imaging and multiparatemer evaluations in parallel, providing the powerful platform for drug evaluation/screening in highthroughput format.
Language英语
WOS IDWOS:000305951500011
Citation statistics
Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/142875
Collection中国科学院大连化学物理研究所
AffiliationChinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
Recommended Citation
GB/T 7714
Wen, Hui,Shi, Weiwei,Qin, Jianhua. Multiparameter evaluation of the longevity in C-elegans under stress using an integrated microfluidic device[J]. BIOMEDICAL MICRODEVICES,2012,14(4):721-728.
APA Wen, Hui,Shi, Weiwei,&Qin, Jianhua.(2012).Multiparameter evaluation of the longevity in C-elegans under stress using an integrated microfluidic device.BIOMEDICAL MICRODEVICES,14(4),721-728.
MLA Wen, Hui,et al."Multiparameter evaluation of the longevity in C-elegans under stress using an integrated microfluidic device".BIOMEDICAL MICRODEVICES 14.4(2012):721-728.
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