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题名: Screening of Metabolite Regulating Lactic Acid Production of Exiguobacterium aurantiacum ATCC49676 by Metabolic Fingerprint Analysis
作者: Lian Rongwei1, 2;  Tian Jing1;  Gao Peng2;  Wang Xiyue2;  Fei Xu1;  Wang Yi1;  Xu Guowang2
关键词: metabolic fingerprint analysis ;  lactic acid ;  GC-MS ;  L-glutamic acid ;  LDH
刊名: ACTA CHIMICA SINICA
发表日期: 2012-12-28
DOI: 10.6023/A12110883
卷: 70, 期:24, 页:2513-2517
收录类别: SCI
文章类型: Article
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
英文摘要: A strain of Exiguobacterium aurantiacum ATCC49676 was found to be of great potential to produce lactic acid (LA). In order to screen the metabolites that might regulate or affect the acid yield, a relative higher LA production condition was defined through full factorial experiment design. Fresh cultures harvested from the basic and the higher LA production conditions were subjected to metabolic fingerprint analysis by gas chromatography-mass spectrometry. Significance analysis indicated a distinct change of an intercellular metabolite-L-glutamic acid (Glu). When ATCC49676 was treated by Glu, LA yield declined with the increasing added Glu concentrations. Relative enzyme quantification confirmed that Glu decreased the intracellular lactate dehydrogenase content. This study proved the merit of metabolic fingerprint analysis in exploring the phenotype specific intracellular metabolite and its potential roles in improving industrial fermentation efficiencies.
关键词[WOS]: MICROBIAL METABOLOMICS ;  FUNCTIONAL GENOMICS ;  MASS-SPECTROMETRY ;  EXTREMOPHILES ;  OPTIMIZATION
语种: 英语
WOS记录号: WOS:000314308100008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142878
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Polytech Univ, Sch Bioengn, Dalian 116034, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Lian Rongwei,Tian Jing,Gao Peng,et al. Screening of Metabolite Regulating Lactic Acid Production of Exiguobacterium aurantiacum ATCC49676 by Metabolic Fingerprint Analysis[J]. ACTA CHIMICA SINICA,2012,70(24):2513-2517.
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