DICP OpenIR
A new reversed-phase/strong anion-exchange mixed-mode stationary phase based on polar-copolymerized approach and its application in the enrichment of aristolochic acids
Wei, Jie1; Guo, Zhimou2; Zhang, Pinjie1; Zhang, Feifang1; Yang, Bingcheng1; Liang, Xinmiao1,2
关键词Solid-phase Extraction Mixed-mode Chromatography Strong Anion Exchange Polar-copolymerized Aristolochic Acids Purification
刊名JOURNAL OF CHROMATOGRAPHY A
2012-07-13
DOI10.1016/j.chroma.2012.03.047
1246页:129-136
收录类别SCI ; ISTP
文章类型Article
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]TANDEM MASS-SPECTROMETRY ; PERFORMANCE LIQUID-CHROMATOGRAPHY ; HUMAN URINE ; ION CHROMATOGRAPHY ; SEPARATION ; EXTRACTION ; PEPTIDES ; CATION ; ARISTOLOLACTAMS ; ARISTOLACTAMS
英文摘要A novel silica-based reversed-phase/strong anion-exchange mixed-mode stationary phase named C18SAX was synthesized based on the polar-copolymerized approach. C18SAX stationary phase showed excellent compatibility with 100% aqueous mobile phase and comparable performance with commercial SunFire (TM) C18 column in terms of column efficiency and methylene selectivity. And the results indicated that hydrophobic interaction and anion-exchange mechanism dominate the separation process of aristolochic acids. The utility of C18SAX material-based SPE was demonstrated for the enrichment of aristolochic acids extracted from Caulis Aristochiae Manshuriensis. The SPE method was developed rapidly and simply by HPLC evaluation. Under the optimized SPE conditions, the average recoveries of aristolochic acid land aristolochic acid II in crude sample were 72.36% and 105.59%, with RSDs of 5.77% and 1.13%, respectively. The complexity of sample matrices was significantly simplified after the SPE procedure and further preparation and purification of target compounds was performed on a preparative C18TDE column. Four aristolochic acids, including aristolochic acid I (31.8 mg), II (8.9 mg), IIIa (6.1 mg) and IVa (4.3 mg) were purified with HPLC purities all above 93%. The results proved that the aristolochic acids could be efficiently enriched by C18SAX material and easily eluted in acidic conditions, indicating its great potential in the enrichment of acidic compounds from complex matrices. (C) 2012 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000306246000021
引用统计
被引频次:35[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/142881
专题中国科学院大连化学物理研究所
作者单位1.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
Wei, Jie,Guo, Zhimou,Zhang, Pinjie,et al. A new reversed-phase/strong anion-exchange mixed-mode stationary phase based on polar-copolymerized approach and its application in the enrichment of aristolochic acids[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1246:129-136.
APA Wei, Jie,Guo, Zhimou,Zhang, Pinjie,Zhang, Feifang,Yang, Bingcheng,&Liang, Xinmiao.(2012).A new reversed-phase/strong anion-exchange mixed-mode stationary phase based on polar-copolymerized approach and its application in the enrichment of aristolochic acids.JOURNAL OF CHROMATOGRAPHY A,1246,129-136.
MLA Wei, Jie,et al."A new reversed-phase/strong anion-exchange mixed-mode stationary phase based on polar-copolymerized approach and its application in the enrichment of aristolochic acids".JOURNAL OF CHROMATOGRAPHY A 1246(2012):129-136.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wei, Jie]的文章
[Guo, Zhimou]的文章
[Zhang, Pinjie]的文章
百度学术
百度学术中相似的文章
[Wei, Jie]的文章
[Guo, Zhimou]的文章
[Zhang, Pinjie]的文章
必应学术
必应学术中相似的文章
[Wei, Jie]的文章
[Guo, Zhimou]的文章
[Zhang, Pinjie]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。