DICP OpenIR
Measurement of pentane in expiratory gas during rabbit hepatic ischemia/reperfusion by solid-phase microextraction and gas chromatography-mass spectrometry (SPME GC/MS)
Liu, Shujuan1; Shi, Jinghui2; Wang, Changsong1; Li, Peng1; Gong, Yulei1; He, Ying1; Xu, Guowang3; Li, Jianyi4; Luo, Ailin2; Li, Enyou1
刊名JOURNAL OF BREATH RESEARCH
2012-06-01
DOI10.1088/1752-7155/6/2/026003
6期:2
收录类别SCI
文章类型Article
类目[WOS]Biochemical Research Methods ; Respiratory System
研究领域[WOS]Biochemistry & Molecular Biology ; Respiratory System
关键词[WOS]ISCHEMIA-REPERFUSION INJURY ; CRITICALLY-ILL PATIENTS ; LIPID-PEROXIDATION ; OXIDATIVE STRESS ; BREATH ; LIVER ; MODEL ; INDICATOR
英文摘要The aim of this study was to determine the changes in the pentane concentration of expiratory gas as well as the relationship between this pentane concentration and hepatic oxidative stress during rabbit hepatic ischemia/reperfusion using solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC/MS). 45 white male rabbits with body weights between 2.5 and 3.0 kg were randomly assigned to the following three groups: the 10 min ischemia group (group S); the 20 min ischemia group (group M); or the 30 min ischemia group (group L). Expiratory gases were collected prior to ischemia (T0) and for 1, 10, 20, 30, 60 and 120 min (T1-T6) following reperfusion. Pentane concentrations were determined using SPME and GC/MS. In addition, arterial blood samples were collected, and serum aminotransferase (AST) and malondialdehyde (MDA) concentrations were measured. In the three groups, the pentane concentrations of the expiratory gases at points T1 and T2 were significantly increased (P < 0.05) compared with those at point T0, and the serum AST and MDA concentrations at points T5 and T6 were also significantly increased (P < 0.05) compared with those at point T0. Therefore, the use of SPME in combination with GC/MS represents an improved anesthesia system that can be used to continuously measure the concentration of pentane in expiratory gases, which can reflect the degree of oxidative stress during hepatic ischemia/reperfusion.
语种英语
WOS记录号WOS:000304411600006
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/142906
专题中国科学院大连化学物理研究所
作者单位1.Harbin Med Univ, Affiliated Hosp 1, Dept Anesthesiol, Harbin, Peoples R China
2.Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Dept Anesthesiol, Wuhan 430074, Peoples R China
3.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian, Peoples R China
4.Third High Sch Harbin, Harbin, Peoples R China
推荐引用方式
GB/T 7714
Liu, Shujuan,Shi, Jinghui,Wang, Changsong,et al. Measurement of pentane in expiratory gas during rabbit hepatic ischemia/reperfusion by solid-phase microextraction and gas chromatography-mass spectrometry (SPME GC/MS)[J]. JOURNAL OF BREATH RESEARCH,2012,6(2).
APA Liu, Shujuan.,Shi, Jinghui.,Wang, Changsong.,Li, Peng.,Gong, Yulei.,...&Li, Enyou.(2012).Measurement of pentane in expiratory gas during rabbit hepatic ischemia/reperfusion by solid-phase microextraction and gas chromatography-mass spectrometry (SPME GC/MS).JOURNAL OF BREATH RESEARCH,6(2).
MLA Liu, Shujuan,et al."Measurement of pentane in expiratory gas during rabbit hepatic ischemia/reperfusion by solid-phase microextraction and gas chromatography-mass spectrometry (SPME GC/MS)".JOURNAL OF BREATH RESEARCH 6.2(2012).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Liu, Shujuan]的文章
[Shi, Jinghui]的文章
[Wang, Changsong]的文章
百度学术
百度学术中相似的文章
[Liu, Shujuan]的文章
[Shi, Jinghui]的文章
[Wang, Changsong]的文章
必应学术
必应学术中相似的文章
[Liu, Shujuan]的文章
[Shi, Jinghui]的文章
[Wang, Changsong]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。