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题名: Preparation of phenyl-silica hybrid monolithic column with "one-pot" process for capillary liquid chromatography
作者: Zhang, Zhenbin1, 2;  Lin, Hui1, 2;  Ou, Junjie1;  Qin, Hongqiang1, 2;  Wu, Ren'an1;  Dong, Jing1;  Zou, Hanfa1
关键词: One-pot ;  Sol-gel ;  Phenyl-silica hybrid monolith ;  Capillary liquid chromatography
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2012-03-09
DOI: 10.1016/j.chroma.2011.07.048
卷: 1228, 页:263-269
收录类别: SCI
文章类型: Article
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: A phenyl-silica hybrid monolithic column for capillary liquid chromatography (cLC) was prepared through "one-pot" process by con-currently using benzyl methacrylate and alkoxysilanes. The effects of the molar ratio of tetramethoxysilane/vinyltrimethoxysilane (TMOS/VTMS), polycondensation temperature, content of supramolecule template (cetyltrimethylammonium bromide, CTAB), ratio of N,N'-dimethylformamide/methanol (v/v), the volume of benzyl methacrylate on the morphologies of the prepared phenyl-silica hybrid monolithic columns were investigated in detail. The permeability of the hybrid monolithic column was calculated as 3.23 x 10(-13) m(2), and the minimum plate height was determined as 8.38 mu m which corresponding to 119,000 theoretical plates per meter. Separation of various neutral, polar and basic analytes as well as small peptides on the hybrid monolithic column was achieved by cLC and showed high efficiency and satisfactory reproducibility. Moreover, the prepared hybrid monolithic column was also applied for the analysis of Cryptic digests of bovine serum albumin (BSA), ovalbumin, a-casein, cytochrome C and myoglobin by cLC tandem mass spectrometry (cLC-MS/MS), and the results showed that the separation performance was close to that of the octadecylsilane (C18) packed capillary column which demonstrating its potential in proteome analysis. Moreover, since the prepolymerization system was mainly consisted of organic solvents (methanol and N,N'-dimethylformamide). various hydrophobic monomers could be potentially used to prepare organic-silica hybrid monolithic columns through "one-pot" approach. (C) 2011 Elsevier B.V. All rights reserved.
关键词[WOS]: IONIZATION MASS-SPECTROMETRY ;  STATIONARY PHASES ;  PROTEOME ANALYSIS ;  INNER DIAMETERS ;  SEPARATION ;  ELECTROCHROMATOGRAPHY ;  EFFICIENCY ;  SINGLE
语种: 英语
WOS记录号: WOS:000301406100026
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142911
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

Recommended Citation:
Zhang, Zhenbin,Lin, Hui,Ou, Junjie,et al. Preparation of phenyl-silica hybrid monolithic column with "one-pot" process for capillary liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2012,1228:263-269.
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