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题名: Global Liver Proteome Analysis Using iTRAQ Labeling Quantitative Proteomic Technology to Reveal Biomarkers in Mice Exposed to Perfluorooctane Sulfonate (PFOS)
作者: Tan, Feng1;  Jin, Yihe1;  Liu, Wei1;  Quan, Xie1;  Chen, Jingwen1;  Liang, Zhen2
刊名: ENVIRONMENTAL SCIENCE & TECHNOLOGY
发表日期: 2012-11-06
DOI: 10.1021/es3027715
卷: 46, 期:21, 页:12170-12177
收录类别: SCI
文章类型: Article
类目[WOS]: Engineering, Environmental ;  Environmental Sciences
研究领域[WOS]: Engineering ;  Environmental Sciences & Ecology
英文摘要: Proteomic analysis allows detection of changes of proteins expression in organisms exposed to environmental pollutants, leading to the discovery of biomarkers of exposure and understanding of the action mechanism of toxicity. In the present study, we applied iTRAQ labeling quantitative proteomic technology for global characterization of the liver proteome in mice exposed to perfluorooctane sulfonate (PFOS). This successfully identified and quantified 1038 unique proteins. Seventy-one proteins showed a significant expression change in the treated groups (1.0, 2.5, 5.0 mg/kg of body weight) compared with the control group, and 16 proteins displayed strong dose dependent changes. Gene ontology analysis showed that these differential proteins were significantly enriched and stimulus. We detected significantly increased expression levels of enzymes regulating mainly involved in lipid metabolism, transport, biosynthetic processes, and response to peroxisomal beta-oxidation-including long chain acyl-CoA synthetase, acyl-CoA oxidase 1, bifunctional enzyme, and 3-ketoacyl-CoA thiolase A. PFOS also significantly induced cytochrome P450s and glutathione S-transferases that are responsible for the metabolism of xenobiotic compounds. The expressions of several proteins with important biological functions-such as cysteine sulfinic acid decarboxylase, aldehyde dehydrogenase, and apolipoprotein A-I, also correlated with PFOS exposure. Together, the present results provide insight into the molecular mechanism and biomarkers for PFOS-induced effects.
关键词[WOS]: GLUTATHIONE S-TRANSFERASES ;  ALPHA TARGET GENES ;  PPAR-ALPHA ;  PERFLUORINATED COMPOUNDS ;  OXIDATIVE STRESS ;  EXPRESSION ;  RATS ;  INDUCTION ;  OVEREXPRESSION ;  METABOLISM
语种: 英语
WOS记录号: WOS:000310665000083
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142917
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Univ Technol, Key Lab Ind Ecol & Environm Engn MOE, Sch Environm Sci & Technol, Dalian 116024, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China

Recommended Citation:
Tan, Feng,Jin, Yihe,Liu, Wei,et al. Global Liver Proteome Analysis Using iTRAQ Labeling Quantitative Proteomic Technology to Reveal Biomarkers in Mice Exposed to Perfluorooctane Sulfonate (PFOS)[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2012,46(21):12170-12177.
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