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Global Liver Proteome Analysis Using iTRAQ Labeling Quantitative Proteomic Technology to Reveal Biomarkers in Mice Exposed to Perfluorooctane Sulfonate (PFOS)
Tan, Feng1; Jin, Yihe1; Liu, Wei1; Quan, Xie1; Chen, Jingwen1; Liang, Zhen2
Source PublicationENVIRONMENTAL SCIENCE & TECHNOLOGY
2012-11-06
DOI10.1021/es3027715
Volume46Issue:21Pages:12170-12177
Indexed BySCI
SubtypeArticle
WOS SubjectEngineering, Environmental ; Environmental Sciences
WOS Research AreaEngineering ; Environmental Sciences & Ecology
WOS KeywordGLUTATHIONE S-TRANSFERASES ; ALPHA TARGET GENES ; PPAR-ALPHA ; PERFLUORINATED COMPOUNDS ; OXIDATIVE STRESS ; EXPRESSION ; RATS ; INDUCTION ; OVEREXPRESSION ; METABOLISM
AbstractProteomic analysis allows detection of changes of proteins expression in organisms exposed to environmental pollutants, leading to the discovery of biomarkers of exposure and understanding of the action mechanism of toxicity. In the present study, we applied iTRAQ labeling quantitative proteomic technology for global characterization of the liver proteome in mice exposed to perfluorooctane sulfonate (PFOS). This successfully identified and quantified 1038 unique proteins. Seventy-one proteins showed a significant expression change in the treated groups (1.0, 2.5, 5.0 mg/kg of body weight) compared with the control group, and 16 proteins displayed strong dose dependent changes. Gene ontology analysis showed that these differential proteins were significantly enriched and stimulus. We detected significantly increased expression levels of enzymes regulating mainly involved in lipid metabolism, transport, biosynthetic processes, and response to peroxisomal beta-oxidation-including long chain acyl-CoA synthetase, acyl-CoA oxidase 1, bifunctional enzyme, and 3-ketoacyl-CoA thiolase A. PFOS also significantly induced cytochrome P450s and glutathione S-transferases that are responsible for the metabolism of xenobiotic compounds. The expressions of several proteins with important biological functions-such as cysteine sulfinic acid decarboxylase, aldehyde dehydrogenase, and apolipoprotein A-I, also correlated with PFOS exposure. Together, the present results provide insight into the molecular mechanism and biomarkers for PFOS-induced effects.
Language英语
WOS IDWOS:000310665000083
Citation statistics
Cited Times:29[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/142917
Collection中国科学院大连化学物理研究所
Affiliation1.Dalian Univ Technol, Key Lab Ind Ecol & Environm Engn MOE, Sch Environm Sci & Technol, Dalian 116024, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Tan, Feng,Jin, Yihe,Liu, Wei,et al. Global Liver Proteome Analysis Using iTRAQ Labeling Quantitative Proteomic Technology to Reveal Biomarkers in Mice Exposed to Perfluorooctane Sulfonate (PFOS)[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2012,46(21):12170-12177.
APA Tan, Feng,Jin, Yihe,Liu, Wei,Quan, Xie,Chen, Jingwen,&Liang, Zhen.(2012).Global Liver Proteome Analysis Using iTRAQ Labeling Quantitative Proteomic Technology to Reveal Biomarkers in Mice Exposed to Perfluorooctane Sulfonate (PFOS).ENVIRONMENTAL SCIENCE & TECHNOLOGY,46(21),12170-12177.
MLA Tan, Feng,et al."Global Liver Proteome Analysis Using iTRAQ Labeling Quantitative Proteomic Technology to Reveal Biomarkers in Mice Exposed to Perfluorooctane Sulfonate (PFOS)".ENVIRONMENTAL SCIENCE & TECHNOLOGY 46.21(2012):12170-12177.
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