中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Development, validation, and application of a liquid chromatography-tandem mass spectrometry method for the determination of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in human hair
作者: Yao, Li1;  Yang, Jun1;  Guan, Ya-feng1, 2;  Liu, Bai-zhan3;  Zheng, Sai-jing3;  Wang, Wei-miao3;  Zhu, Xiao-lan1;  Zhang, Zhi-dan1
关键词: 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol ;  Molecularly imprinted polymers ;  Solid-phase extraction ;  Dispersive liquid-liquid microextraction ;  Liquid chromatography tandem mass spectrometry ;  Human hair
刊名: ANALYTICAL AND BIOANALYTICAL CHEMISTRY
发表日期: 2012-11-01
DOI: 10.1007/s00216-012-6356-1
卷: 404, 期:8, 页:2259-2266
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: The tobacco-specific nitrosamine metabolite 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) is a valuable biomarker for human exposure to the carcinogenic nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in tobacco and tobacco smoke. In this work, an efficient and sensitive method for the analysis of NNAL in human hair was developed and validated. The hair sample was extracted by NaOH solution digestion, purified by C-18 solid-phase extraction (SPE) and molecularly imprinted solid-phase extraction, further enriched by reverse-phase ultrasound-assisted dispersive liquid-liquid microextraction (USA-DLLME) into 1.0 % aqueous formic acid, and finally analyzed by liquid chromatography-electrospray ionization tandem mass spectrometry. Good linearity was obtained in the range of 0.24-10.0 pg/mg hair with a correlation coefficient of 0.9982, when 150 mg hair was analyzed. The limit of detection and lower limit of quantification were 0.08 and 0.24 pg/mg hair, respectively. Accuracies determined from hair samples spiked with three different levels of NNAL ranged between 87.3 and 107.7 %. Intra- and inter-day relative standard deviations varied from 4.1 to 8.5 % and from 6.9 to 11.3 %, respectively. Under the optimized conditions, an enrichment factor of 20 was obtained. Finally, the developed method was applied for the analysis of NNAL in smokers' hair. The proposed sample preparation procedure combining selectivity of two-step SPE and enrichment of DLLME significantly improves the purification and enrichment of the analyte and should be useful to analyze NNAL in hair samples for cancer risk evaluation and cancer prevention in relation to exposure to the tobacco-specific carcinogen NNK.
关键词[WOS]: TOBACCO-SPECIFIC NITROSAMINE ;  SOLID-PHASE EXTRACTION ;  MOLECULARLY IMPRINTED POLYMER ;  HUMAN URINE ;  SELECTIVE EXTRACTION ;  GAS-CHROMATOGRAPHY ;  ORGANIC-COMPOUNDS ;  IN-VITRO ;  MICROEXTRACTION ;  PRECONCENTRATION
语种: 英语
WOS记录号: WOS:000310083900018
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142975
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Univ Sci & Technol China, Dept Chem, Hefei 230026, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Microinstrumentat & Analyt Chem, Dalian 116023, Peoples R China
3.China Natl Tobacco Corp, Key Lab Cigarette Smoke Res, Shanghai 200082, Peoples R China

Recommended Citation:
Yao, Li,Yang, Jun,Guan, Ya-feng,et al. Development, validation, and application of a liquid chromatography-tandem mass spectrometry method for the determination of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in human hair[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2012,404(8):2259-2266.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Yao, Li]'s Articles
 [Yang, Jun]'s Articles
 [Guan, Ya-feng]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Yao, Li]‘s Articles
 [Yang, Jun]‘s Articles
 [Guan, Ya-feng]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace