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Development, validation, and application of a liquid chromatography-tandem mass spectrometry method for the determination of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in human hair
Yao, Li1; Yang, Jun1; Guan, Ya-feng1,2; Liu, Bai-zhan3; Zheng, Sai-jing3; Wang, Wei-miao3; Zhu, Xiao-lan1; Zhang, Zhi-dan1
关键词4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol Molecularly Imprinted Polymers Solid-phase Extraction Dispersive Liquid-liquid Microextraction Liquid Chromatography Tandem Mass Spectrometry Human Hair
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
2012-11-01
DOI10.1007/s00216-012-6356-1
404期:8页:2259-2266
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]TOBACCO-SPECIFIC NITROSAMINE ; SOLID-PHASE EXTRACTION ; MOLECULARLY IMPRINTED POLYMER ; HUMAN URINE ; SELECTIVE EXTRACTION ; GAS-CHROMATOGRAPHY ; ORGANIC-COMPOUNDS ; IN-VITRO ; MICROEXTRACTION ; PRECONCENTRATION
英文摘要The tobacco-specific nitrosamine metabolite 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) is a valuable biomarker for human exposure to the carcinogenic nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in tobacco and tobacco smoke. In this work, an efficient and sensitive method for the analysis of NNAL in human hair was developed and validated. The hair sample was extracted by NaOH solution digestion, purified by C-18 solid-phase extraction (SPE) and molecularly imprinted solid-phase extraction, further enriched by reverse-phase ultrasound-assisted dispersive liquid-liquid microextraction (USA-DLLME) into 1.0 % aqueous formic acid, and finally analyzed by liquid chromatography-electrospray ionization tandem mass spectrometry. Good linearity was obtained in the range of 0.24-10.0 pg/mg hair with a correlation coefficient of 0.9982, when 150 mg hair was analyzed. The limit of detection and lower limit of quantification were 0.08 and 0.24 pg/mg hair, respectively. Accuracies determined from hair samples spiked with three different levels of NNAL ranged between 87.3 and 107.7 %. Intra- and inter-day relative standard deviations varied from 4.1 to 8.5 % and from 6.9 to 11.3 %, respectively. Under the optimized conditions, an enrichment factor of 20 was obtained. Finally, the developed method was applied for the analysis of NNAL in smokers' hair. The proposed sample preparation procedure combining selectivity of two-step SPE and enrichment of DLLME significantly improves the purification and enrichment of the analyte and should be useful to analyze NNAL in hair samples for cancer risk evaluation and cancer prevention in relation to exposure to the tobacco-specific carcinogen NNK.
语种英语
WOS记录号WOS:000310083900018
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/142975
专题中国科学院大连化学物理研究所
作者单位1.Univ Sci & Technol China, Dept Chem, Hefei 230026, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Microinstrumentat & Analyt Chem, Dalian 116023, Peoples R China
3.China Natl Tobacco Corp, Key Lab Cigarette Smoke Res, Shanghai 200082, Peoples R China
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GB/T 7714
Yao, Li,Yang, Jun,Guan, Ya-feng,et al. Development, validation, and application of a liquid chromatography-tandem mass spectrometry method for the determination of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in human hair[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2012,404(8):2259-2266.
APA Yao, Li.,Yang, Jun.,Guan, Ya-feng.,Liu, Bai-zhan.,Zheng, Sai-jing.,...&Zhang, Zhi-dan.(2012).Development, validation, and application of a liquid chromatography-tandem mass spectrometry method for the determination of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in human hair.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,404(8),2259-2266.
MLA Yao, Li,et al."Development, validation, and application of a liquid chromatography-tandem mass spectrometry method for the determination of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in human hair".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 404.8(2012):2259-2266.
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