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Comprehensive and Highly Sensitive Urinary Steroid Hormone Profiling Method Based on Stable Isotope-Labeling Liquid Chromatography Mass Spectrometry
Dai, Weidong; Huang, Qiang; Yin, Peiyuan; Li, Jia; Zhou, Jia; Kong, Hongwei; Zhao, Chunxia; Lu, Xin; Xu, Guowang
刊名ANALYTICAL CHEMISTRY
2012-12-04
DOI10.1021/ac301984t
84期:23页:10245-10251
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]PROSTATE-CANCER ; BREAST-CANCER ; RELATIVE QUANTIFICATION ; QUANTITATIVE PROTEOMICS ; CONTAINING METABOLITES ; RHEUMATOID-ARTHRITIS ; ENDOGENOUS ESTROGENS ; HUMAN SERUM ; DERIVATIZATION ; RISK
英文摘要Steroid hormones are crucial substances that mediate a wide range of vital physiological functions of the body. Determination of the levels of steroid hormones plays an important role in understanding the mechanism of the steroid hormone-related diseases. In this study, we present a novel targeted metabolic profiling method based on the introduction of an easily protonated stable isotope tag to a hydroxyl-containing steroid hormone with a synthesized derivatization reagent, deuterium 4-(dimethylamino)-benzoic acid (d(4)-DMBA), and liquid chromatography mass spectrometry (LC-MS). Different from other reported derivatization reagents that have been used to enhance the sensitivities for estrogens or androgens, our method is comprehensive with the capability of covering hydroxyl-containing androgens, estrogens, corticoids, and progestogens. Furthermore, the nonderivatized steroid hormones (e.g., 17 alpha-hydroxyprogesterone, progesterone, and androstenedione) were not destroyed during the derivatization process, and their levels could still be obtained in one LC-MS run. We were able to detect 2.4 steroid hormones at subng/mL levels (the lower limit of detection could reach 5 pg/mL for estrone and 16 alpha-hydroxy estrone, which is equivalent to 0.1 pg on column) with maximum sensitivity enhancement factors of more than 10(3)- to 10(4)-fold after derivatization. The method was successfully applied to the measurement of free (unconjugated) steroid hormones in urine samples of males, females, and pregnant women. Because the significant role the steroid hormone pathway plays in humans, a comprehensive, sensitive, specific, and accurate method for profiling the steroid hormone metabolome shall offer new insights into hormone-related diseases.
语种英语
WOS记录号WOS:000311815300016
引用统计
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/142980
专题中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
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GB/T 7714
Dai, Weidong,Huang, Qiang,Yin, Peiyuan,et al. Comprehensive and Highly Sensitive Urinary Steroid Hormone Profiling Method Based on Stable Isotope-Labeling Liquid Chromatography Mass Spectrometry[J]. ANALYTICAL CHEMISTRY,2012,84(23):10245-10251.
APA Dai, Weidong.,Huang, Qiang.,Yin, Peiyuan.,Li, Jia.,Zhou, Jia.,...&Xu, Guowang.(2012).Comprehensive and Highly Sensitive Urinary Steroid Hormone Profiling Method Based on Stable Isotope-Labeling Liquid Chromatography Mass Spectrometry.ANALYTICAL CHEMISTRY,84(23),10245-10251.
MLA Dai, Weidong,et al."Comprehensive and Highly Sensitive Urinary Steroid Hormone Profiling Method Based on Stable Isotope-Labeling Liquid Chromatography Mass Spectrometry".ANALYTICAL CHEMISTRY 84.23(2012):10245-10251.
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