中国科学院大连化学物理研究所机构知识库
Advanced  
DICP OpenIR  > 中国科学院大连化学物理研究所  > 期刊论文
题名: Comprehensive and Highly Sensitive Urinary Steroid Hormone Profiling Method Based on Stable Isotope-Labeling Liquid Chromatography Mass Spectrometry
作者: Dai, Weidong1;  Huang, Qiang1;  Yin, Peiyuan1;  Li, Jia1;  Zhou, Jia1;  Kong, Hongwei1;  Zhao, Chunxia1;  Lu, Xin1;  Xu, Guowang1
刊名: ANALYTICAL CHEMISTRY
发表日期: 2012-12-04
DOI: 10.1021/ac301984t
卷: 84, 期:23, 页:10245-10251
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Steroid hormones are crucial substances that mediate a wide range of vital physiological functions of the body. Determination of the levels of steroid hormones plays an important role in understanding the mechanism of the steroid hormone-related diseases. In this study, we present a novel targeted metabolic profiling method based on the introduction of an easily protonated stable isotope tag to a hydroxyl-containing steroid hormone with a synthesized derivatization reagent, deuterium 4-(dimethylamino)-benzoic acid (d(4)-DMBA), and liquid chromatography mass spectrometry (LC-MS). Different from other reported derivatization reagents that have been used to enhance the sensitivities for estrogens or androgens, our method is comprehensive with the capability of covering hydroxyl-containing androgens, estrogens, corticoids, and progestogens. Furthermore, the nonderivatized steroid hormones (e.g., 17 alpha-hydroxyprogesterone, progesterone, and androstenedione) were not destroyed during the derivatization process, and their levels could still be obtained in one LC-MS run. We were able to detect 2.4 steroid hormones at subng/mL levels (the lower limit of detection could reach 5 pg/mL for estrone and 16 alpha-hydroxy estrone, which is equivalent to 0.1 pg on column) with maximum sensitivity enhancement factors of more than 10(3)- to 10(4)-fold after derivatization. The method was successfully applied to the measurement of free (unconjugated) steroid hormones in urine samples of males, females, and pregnant women. Because the significant role the steroid hormone pathway plays in humans, a comprehensive, sensitive, specific, and accurate method for profiling the steroid hormone metabolome shall offer new insights into hormone-related diseases.
关键词[WOS]: PROSTATE-CANCER ;  BREAST-CANCER ;  RELATIVE QUANTIFICATION ;  QUANTITATIVE PROTEOMICS ;  CONTAINING METABOLITES ;  RHEUMATOID-ARTHRITIS ;  ENDOGENOUS ESTROGENS ;  HUMAN SERUM ;  DERIVATIZATION ;  RISK
语种: 英语
WOS记录号: WOS:000311815300016
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142980
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China

Recommended Citation:
Dai, Weidong,Huang, Qiang,Yin, Peiyuan,et al. Comprehensive and Highly Sensitive Urinary Steroid Hormone Profiling Method Based on Stable Isotope-Labeling Liquid Chromatography Mass Spectrometry[J]. ANALYTICAL CHEMISTRY,2012,84(23):10245-10251.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Dai, Weidong]'s Articles
 [Huang, Qiang]'s Articles
 [Yin, Peiyuan]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Dai, Weidong]‘s Articles
 [Huang, Qiang]‘s Articles
 [Yin, Peiyuan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace