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题名: Aptamer Modified Organic-Inorganic Hybrid Silica Monolithic Capillary Columns for Highly Selective Recognition of Thrombin
作者: Deng, Nan1, 2;  Liang, Zhen1;  Liang, Yu1;  Sui, Zhigang1;  Zhang, Liyuan1, 2;  Wu, Qi1, 2;  Yang, Kaiguang1;  Zhang, Lihua1;  Zhang, Yukui1
刊名: ANALYTICAL CHEMISTRY
发表日期: 2012-12-04
DOI: 10.1021/ac302779u
卷: 84, 期:23, 页:10186-10190
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: A novel kind of aptamer modified organic-inorganic hybrid silica monolithic capillary column has been developed, via the covalent bonding of 5'-NH2-modified aptamer for human alpha-thrombin on hybrid silica monolith, prepared by sol-gel method, with tetraethoxysilane and 3-aminopropyltriethoxysilane as precursors. Due to the large specific surface area of the hybrid matrix, the average coverage density of aptamer reached 568 pmol/mu L, and the thrombin binding capacity was 1.15 mu g/mu L, 14 times higher than that of aptamer modified open tubular capillaries. By such an affinity capillary column, the limit of detection of thrombin was decreased to 3.4 nM with a UV detector. Furthermore, even when thrombin was mixed with 1000 times more concentrated human serum, it could be selectively enriched and detected with the signal-to-noise ratio as ca.10. These results indicate that the developed preparation strategy for aptamer based hybrid silica monolithic capillary column might provide an effective method to achieve highly selective recognition of trace targets.
关键词[WOS]: GEL-DERIVED SILICA ;  STATIONARY-PHASE ;  AFFINITY-CHROMATOGRAPHY ;  LIQUID-CHROMATOGRAPHY ;  MAGNETIC BEADS ;  DNA APTAMERS ;  SEPARATION ;  ADENOSINE ;  PROTEINS ;  ELECTROCHROMATOGRAPHY
语种: 英语
WOS记录号: WOS:000311815300008
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/142981
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China

Recommended Citation:
Deng, Nan,Liang, Zhen,Liang, Yu,et al. Aptamer Modified Organic-Inorganic Hybrid Silica Monolithic Capillary Columns for Highly Selective Recognition of Thrombin[J]. ANALYTICAL CHEMISTRY,2012,84(23):10186-10190.
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