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题名: Magnetic nanoparticles: An improved method for mitochondrial isolation
作者: Tang, Bo1;  Zhao, Liang2;  Liang, Rui1;  Zhang, Yi1;  Wang, Liming1
关键词: magnetic nanoparticles ;  mitochondria ;  isolation
刊名: MOLECULAR MEDICINE REPORTS
发表日期: 2012-05-01
DOI: 10.3892/mmr.2012.806
卷: 5, 期:5, 页:1271-1276
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Oncology ;  Medicine, Research & Experimental
研究领域[WOS]: Oncology ;  Research & Experimental Medicine
英文摘要: The ultrastructure, function, and physical and chemical properties of mitochondria have become important issues in scientific research. Current mitochondrial isolation methods mainly rely on the physical and chemical properties of mitochondria. Our team presents a fast and reliable new isolation method based on magnetic nanoparticles binding with monoamine oxidase-A (MAO-A) expressed in the mitochondria. MAO-A is expressed in the outer membrane of human mitochondria and is localized on the cytoplasmic side of the membrane, which makes it possible to isolate the mitochondria effectively, using a magnetic field. As shown in the present study, in comparison with differential centrifugation and density gradient centrifugation, the yield of mitochondria isolated by magnetic nanoparticle binding is higher, with greater mitochondrial purity and activity. The entire process, from cell harvesting to final isolation of the mitochondria, takes approximately 1 h. Magnetic nanoparticles provide a simple, practical approach for mitochondrial isolation.
关键词[WOS]: PROTEIN EXPRESSION ;  NITRIC-OXIDE ;  APOPTOSIS ;  DYSFUNCTION ;  CELLS ;  LIVER ;  CARDIOLIPIN ;  SENESCENCE ;  PROTEOMICS ;  BRAIN
语种: 英语
WOS记录号: WOS:000303281700027
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143031
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Dalian Med Univ, Affiliated Hosp 2, Dept Gen Surg, Dalian 116027, Liaoning, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China

Recommended Citation:
Tang, Bo,Zhao, Liang,Liang, Rui,et al. Magnetic nanoparticles: An improved method for mitochondrial isolation[J]. MOLECULAR MEDICINE REPORTS,2012,5(5):1271-1276.
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