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题名: Metabolic profiling of transgenic rice progeny using gas chromatography-mass spectrometry: the effects of gene insertion, tissue culture and breeding
作者: Zhou, Jia1;  Zhang, Lei2, 3;  Li, Xiang1;  Chang, Yuwei1;  Gu, Qun1;  Lu, Xin1;  Zhu, Zhen2, 3;  Xu, Guowang1
关键词: Transgenic rice ;  Gene insertion ;  Tissue culture ;  Traditional breeding ;  Metabolic profiling ;  GC-MS
刊名: METABOLOMICS
发表日期: 2012-08-01
DOI: 10.1007/s11306-011-0338-8
卷: 8, 期:4, 页:529-539
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Endocrinology & Metabolism
研究领域[WOS]: Endocrinology & Metabolism
英文摘要: The Bacillus thuringiensis delta-endotoxin and cowpea trypsin inhibitor genes have been introduced into the rice genome to improve its pest resistance via Agrobacterium-mediated transformation. A gas chromatography-mass spectrometry (GC-MS) based metabolic profiling method was employed to determine the unpredictable metabolic changes resulting from the gene insertion and tissue culture separately. Descendants of the same transformant were obtained from different breeding programs, including both the transgenic and null-segregant progeny. The comparison of the transgenic and respective null-segregant plants enabled the evaluation of variations caused by transgenes; also the null-segregant plants were compared with the wild-type control to identify the influence of tissue culture. Based on the GC-MS metabolic profiles, the principal component analysis and significant differences determined by Student's t-test suggested that there were more metabolic changes from the tissue culture than those from the insertion of the transgenes. By comparing different breeding programs, it was clear that the progeny which was developed after several generations of backcross with the non-transformed rice as the recurrent parent, displayed fewer metabolic differences from the non-transformed parent. A GC-MS based metabolic profiling study confirmed that backcrossing can help to reduce unwanted variations that occur during transformation processes.
关键词[WOS]: BACILLUS-THURINGIENSIS ;  SOMACLONAL VARIATION ;  INSECT RESISTANCE ;  METABONOMIC ANALYSIS ;  FIELD PERFORMANCE ;  TOBACCO PLANTS ;  HPLC-MS ;  POTATO ;  ARABIDOPSIS ;  TUBER
语种: 英语
WOS记录号: WOS:000306549600001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143072
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China
3.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res, Beijing 100101, Peoples R China

Recommended Citation:
Zhou, Jia,Zhang, Lei,Li, Xiang,et al. Metabolic profiling of transgenic rice progeny using gas chromatography-mass spectrometry: the effects of gene insertion, tissue culture and breeding[J]. METABOLOMICS,2012,8(4):529-539.
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