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题名: Characterization of the mitochondrial NAD(+)-dependent isocitrate dehydrogenase of the oleaginous yeast Rhodosporidium toruloides
作者: Yang, Fan1, 2;  Zhang, Sufang1;  Zhou, Yongjin J.1;  Zhu, Zhiwei1;  Lin, Xinping1;  Zhao, Zongbao K.1, 3
关键词: Rhodosporidium toruloides ;  Microbial lipid ;  NAD(+)-specific isocitrate dehydrogenase ;  Nitrogen limitation ;  Oleaginous yeast
刊名: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
发表日期: 2012-05-01
DOI: 10.1007/s00253-011-3820-3
卷: 94, 期:4, 页:1095-1105
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine
类目[WOS]: Biotechnology & Applied Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology
英文摘要: Early biochemical studies have demonstrated that lipid accumulation by oleaginous yeasts is linked to the activity of the NAD(+)-dependent isocitrate dehydrogenase (Idh). However, molecular study of Idh of oleaginous microorganisms remains limited. Here, we present the cloning of a mitochondrial NAD(+)-specific Idh from Rhodosporidium toruloides (RtIdh), an excellent microbial lipid producer that uses carbohydrates as the carbon source. The evolutionary relationship analyses among RtIdhs and other yeast Idhs revealed that RtIdh had a closer relationship with the Idhs of Ustilago maydis and Schizophyllum commune. We expressed the RtIDH gene in the yeast Saccharomyces cerevisiae idh Delta mutant. Under the nitrogen-limited condition, the intracellular lipid content and extracellular citrate concentration of the culture of the S. cerevisiae idh Delta carrying the RtIDH gene increased as the carbon/nitrogen molar ratio of the media increased, while the wild-type S. cerevisiae strain showed no correlation. Our data provided valuable information for elucidating the molecular mechanism of microbial oleaginicity and for engineering microorganisms to produce metabolites of fatty acid pathway.
关键词[WOS]: LIPID-ACCUMULATION ;  SACCHAROMYCES-CEREVISIAE ;  BINDING-SITES ;  YARROWIA-LIPOLYTICA ;  GENE ;  ACID ;  DISRUPTION ;  METABOLISM ;  FRAGMENTS ;  PRODUCTS
语种: 英语
WOS记录号: WOS:000303544400024
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143085
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Div Biotechnol, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
2.Dalian Polytech Univ, Sch Biol Engn, Dalian 116034, Peoples R China
3.Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China

Recommended Citation:
Yang, Fan,Zhang, Sufang,Zhou, Yongjin J.,et al. Characterization of the mitochondrial NAD(+)-dependent isocitrate dehydrogenase of the oleaginous yeast Rhodosporidium toruloides[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2012,94(4):1095-1105.
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