DICP OpenIR
Urinary hydrophilic and hydrophobic metabolic profiling based on liquid chromatography-mass spectrometry methods: Differential metabolite discovery specific to ovarian cancer
Chen, Jing1; Zhou, Lina1; Zhang, Xiaoyan2,3; Lu, Xin1; Cao, Rui4; Xu, Congjian2,3; Xu, Guowang1
KeywordHydrophilic Interaction Chromatography Lc-ms Metabolomics Ovarian Cancer Urine
Source PublicationELECTROPHORESIS
2012-11-01
DOI10.1002/elps.201200140
Volume33Issue:22Pages:3361-3369
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
WOS KeywordHEPATOCELLULAR-CARCINOMA ; METABONOMIC ANALYSIS ; LUNG-CANCER ; RAT URINE ; BIOMARKERS ; MS ; DIAGNOSIS ; IDENTIFICATION ; MARKER ; HEALTH
AbstractDiscovery of novel metabolite biomarker(s) for improved ovarian cancer diagnosis is of great importance. In this paper, the differences of urinary hydrophilic and hydrophobic metabolic profiling between healthy women, benign ovarian tumor, and ovarian cancer patients were studied by metabolomics strategy. Metabolites in urine were analyzed on hydrophilic interaction chromatography (HILIC) and reversed-phase liquid chromatography (RPLC) coupled to MS. Data from HILIC or RPLC, positive or negative ion detection mode were found to be complementary. Data were filtered by orthogonal signal correction (OSC) method, and the three groups were discriminated by partial least squares discrimination analysis (PLS-DA) models. By combining the four datasets, maximum information can be collected, and a PLS-DA model was built after OSC filtering. The model based on combined dataset is superior to the ones based on the separate dataset, and important metabolites were screened based on the combined dataset model. Five metabolites were found to be specific to ovarian cancer and ten metabolites were considered commonly related to ovarian cancer and benign ovarian tumor. Combination of RPLC and HILIC separation, as well as positive and negative ion detection in metabolomic studies show advantages in collecting various metabolites information that helps us better understand the metabolic event.
Language英语
WOS IDWOS:000311303100015
Citation statistics
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/143117
Collection中国科学院大连化学物理研究所
Affiliation1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Fudan Univ, Inst Biomed Sci, Shanghai Med Sch, Obstet & Gynecol Hosp, Shanghai 200433, Peoples R China
3.Shanghai Key Lab Female Reprod Endocrine Related, Shanghai, Peoples R China
4.Dalian Med Univ, Hosp Dalian, Dept Obstet & Gynecol, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Chen, Jing,Zhou, Lina,Zhang, Xiaoyan,et al. Urinary hydrophilic and hydrophobic metabolic profiling based on liquid chromatography-mass spectrometry methods: Differential metabolite discovery specific to ovarian cancer[J]. ELECTROPHORESIS,2012,33(22):3361-3369.
APA Chen, Jing.,Zhou, Lina.,Zhang, Xiaoyan.,Lu, Xin.,Cao, Rui.,...&Xu, Guowang.(2012).Urinary hydrophilic and hydrophobic metabolic profiling based on liquid chromatography-mass spectrometry methods: Differential metabolite discovery specific to ovarian cancer.ELECTROPHORESIS,33(22),3361-3369.
MLA Chen, Jing,et al."Urinary hydrophilic and hydrophobic metabolic profiling based on liquid chromatography-mass spectrometry methods: Differential metabolite discovery specific to ovarian cancer".ELECTROPHORESIS 33.22(2012):3361-3369.
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