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Urinary hydrophilic and hydrophobic metabolic profiling based on liquid chromatography-mass spectrometry methods: Differential metabolite discovery specific to ovarian cancer
Chen, Jing1; Zhou, Lina1; Zhang, Xiaoyan2,3; Lu, Xin1; Cao, Rui4; Xu, Congjian2,3; Xu, Guowang1
关键词Hydrophilic Interaction Chromatography Lc-ms Metabolomics Ovarian Cancer Urine
刊名ELECTROPHORESIS
2012-11-01
DOI10.1002/elps.201200140
33期:22页:3361-3369
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]HEPATOCELLULAR-CARCINOMA ; METABONOMIC ANALYSIS ; LUNG-CANCER ; RAT URINE ; BIOMARKERS ; MS ; DIAGNOSIS ; IDENTIFICATION ; MARKER ; HEALTH
英文摘要Discovery of novel metabolite biomarker(s) for improved ovarian cancer diagnosis is of great importance. In this paper, the differences of urinary hydrophilic and hydrophobic metabolic profiling between healthy women, benign ovarian tumor, and ovarian cancer patients were studied by metabolomics strategy. Metabolites in urine were analyzed on hydrophilic interaction chromatography (HILIC) and reversed-phase liquid chromatography (RPLC) coupled to MS. Data from HILIC or RPLC, positive or negative ion detection mode were found to be complementary. Data were filtered by orthogonal signal correction (OSC) method, and the three groups were discriminated by partial least squares discrimination analysis (PLS-DA) models. By combining the four datasets, maximum information can be collected, and a PLS-DA model was built after OSC filtering. The model based on combined dataset is superior to the ones based on the separate dataset, and important metabolites were screened based on the combined dataset model. Five metabolites were found to be specific to ovarian cancer and ten metabolites were considered commonly related to ovarian cancer and benign ovarian tumor. Combination of RPLC and HILIC separation, as well as positive and negative ion detection in metabolomic studies show advantages in collecting various metabolites information that helps us better understand the metabolic event.
语种英语
WOS记录号WOS:000311303100015
引用统计
被引频次:32[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/143117
专题中国科学院大连化学物理研究所
作者单位1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Fudan Univ, Inst Biomed Sci, Shanghai Med Sch, Obstet & Gynecol Hosp, Shanghai 200433, Peoples R China
3.Shanghai Key Lab Female Reprod Endocrine Related, Shanghai, Peoples R China
4.Dalian Med Univ, Hosp Dalian, Dept Obstet & Gynecol, Dalian, Peoples R China
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GB/T 7714
Chen, Jing,Zhou, Lina,Zhang, Xiaoyan,et al. Urinary hydrophilic and hydrophobic metabolic profiling based on liquid chromatography-mass spectrometry methods: Differential metabolite discovery specific to ovarian cancer[J]. ELECTROPHORESIS,2012,33(22):3361-3369.
APA Chen, Jing.,Zhou, Lina.,Zhang, Xiaoyan.,Lu, Xin.,Cao, Rui.,...&Xu, Guowang.(2012).Urinary hydrophilic and hydrophobic metabolic profiling based on liquid chromatography-mass spectrometry methods: Differential metabolite discovery specific to ovarian cancer.ELECTROPHORESIS,33(22),3361-3369.
MLA Chen, Jing,et al."Urinary hydrophilic and hydrophobic metabolic profiling based on liquid chromatography-mass spectrometry methods: Differential metabolite discovery specific to ovarian cancer".ELECTROPHORESIS 33.22(2012):3361-3369.
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