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题名: Urinary hydrophilic and hydrophobic metabolic profiling based on liquid chromatography-mass spectrometry methods: Differential metabolite discovery specific to ovarian cancer
作者: Chen, Jing1;  Zhou, Lina1;  Zhang, Xiaoyan2, 3;  Lu, Xin1;  Cao, Rui4;  Xu, Congjian2, 3;  Xu, Guowang1
关键词: Hydrophilic interaction chromatography ;  LC-MS ;  Metabolomics ;  Ovarian cancer ;  Urine
刊名: ELECTROPHORESIS
发表日期: 2012-11-01
DOI: 10.1002/elps.201200140
卷: 33, 期:22, 页:3361-3369
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Life Sciences & Biomedicine ;  Physical Sciences
类目[WOS]: Biochemical Research Methods ;  Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ;  Chemistry
英文摘要: Discovery of novel metabolite biomarker(s) for improved ovarian cancer diagnosis is of great importance. In this paper, the differences of urinary hydrophilic and hydrophobic metabolic profiling between healthy women, benign ovarian tumor, and ovarian cancer patients were studied by metabolomics strategy. Metabolites in urine were analyzed on hydrophilic interaction chromatography (HILIC) and reversed-phase liquid chromatography (RPLC) coupled to MS. Data from HILIC or RPLC, positive or negative ion detection mode were found to be complementary. Data were filtered by orthogonal signal correction (OSC) method, and the three groups were discriminated by partial least squares discrimination analysis (PLS-DA) models. By combining the four datasets, maximum information can be collected, and a PLS-DA model was built after OSC filtering. The model based on combined dataset is superior to the ones based on the separate dataset, and important metabolites were screened based on the combined dataset model. Five metabolites were found to be specific to ovarian cancer and ten metabolites were considered commonly related to ovarian cancer and benign ovarian tumor. Combination of RPLC and HILIC separation, as well as positive and negative ion detection in metabolomic studies show advantages in collecting various metabolites information that helps us better understand the metabolic event.
关键词[WOS]: HEPATOCELLULAR-CARCINOMA ;  METABONOMIC ANALYSIS ;  LUNG-CANCER ;  RAT URINE ;  BIOMARKERS ;  MS ;  DIAGNOSIS ;  IDENTIFICATION ;  MARKER ;  HEALTH
语种: 英语
WOS记录号: WOS:000311303100015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143117
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
2.Fudan Univ, Inst Biomed Sci, Shanghai Med Sch, Obstet & Gynecol Hosp, Shanghai 200433, Peoples R China
3.Shanghai Key Lab Female Reprod Endocrine Related, Shanghai, Peoples R China
4.Dalian Med Univ, Hosp Dalian, Dept Obstet & Gynecol, Dalian, Peoples R China

Recommended Citation:
Chen, Jing,Zhou, Lina,Zhang, Xiaoyan,et al. Urinary hydrophilic and hydrophobic metabolic profiling based on liquid chromatography-mass spectrometry methods: Differential metabolite discovery specific to ovarian cancer[J]. ELECTROPHORESIS,2012,33(22):3361-3369.
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