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Comprehensive characterization of Stevia Rebaudiana using two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography
Fu, Qing1,2; Guo, Zhimou2; Zhang, Xiuli2; Liu, Yanfang2; Liang, Xinmiao1,2
关键词Characterization Hilic Rp Stevia Rebaudiana Two-dimensional Liquid Chromatography (2d-lc)
刊名JOURNAL OF SEPARATION SCIENCE
2012-07-01
DOI10.1002/jssc.201101103
35期:14页:1821-1827
收录类别SCI
文章类型Article
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]MASS-SPECTROMETRY ; FLUID EXTRACTION ; PLANT-ORIGIN ; STEVIOSIDE ; GLYCOSIDES ; SEPARATION ; PEPTIDES ; LEAVES ; SWEETENERS ; BERTONI
英文摘要Two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography (2D-RPLC/HILIC) system was successfully applied for comprehensive characterization of steviol glycosides from Stevia rebaudiana. The experiments were performed in offline mode using an XCharge C18 column in first dimension and an XAmide column in second dimension. In first dimension, preliminary separation of Stevia aqueous extract was accomplished and 30 fractions were collected. Then fractions 120 were selected for further purification and 13 compounds with high purity were obtained in second dimension. Comprehensive characterization of these compounds was completed by determination of their retention time, accurate molecular weight, diagnostic fragmentation ions, and nuclear magnetic resonance spectroscopy. As a result, all nine known steviol glycosides, as well as other four steviol glycosides were fully purified. The result demonstrated that this procedure is an effective approach for the preparative separation and comprehensive characterization of steviol glycosides in Stevia. This 2D-RPLC/HILIC method will be a promising tool for the purification of low-abundance compounds from natural products.
语种英语
WOS记录号WOS:000306493700015
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文献类型期刊论文
条目标识符http://cas-ir.dicp.ac.cn/handle/321008/143118
专题中国科学院大连化学物理研究所
作者单位1.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Nat Med Liaoning Prov, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
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GB/T 7714
Fu, Qing,Guo, Zhimou,Zhang, Xiuli,et al. Comprehensive characterization of Stevia Rebaudiana using two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2012,35(14):1821-1827.
APA Fu, Qing,Guo, Zhimou,Zhang, Xiuli,Liu, Yanfang,&Liang, Xinmiao.(2012).Comprehensive characterization of Stevia Rebaudiana using two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography.JOURNAL OF SEPARATION SCIENCE,35(14),1821-1827.
MLA Fu, Qing,et al."Comprehensive characterization of Stevia Rebaudiana using two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography".JOURNAL OF SEPARATION SCIENCE 35.14(2012):1821-1827.
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