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Comprehensive characterization of Stevia Rebaudiana using two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography
Fu, Qing1,2; Guo, Zhimou2; Zhang, Xiuli2; Liu, Yanfang2; Liang, Xinmiao1,2
KeywordCharacterization Hilic Rp Stevia Rebaudiana Two-dimensional Liquid Chromatography (2d-lc)
Source PublicationJOURNAL OF SEPARATION SCIENCE
2012-07-01
DOI10.1002/jssc.201101103
Volume35Issue:14Pages:1821-1827
Indexed BySCI
SubtypeArticle
WOS HeadingsScience & Technology ; Physical Sciences
WOS SubjectChemistry, Analytical
WOS Research AreaChemistry
WOS KeywordMASS-SPECTROMETRY ; FLUID EXTRACTION ; PLANT-ORIGIN ; STEVIOSIDE ; GLYCOSIDES ; SEPARATION ; PEPTIDES ; LEAVES ; SWEETENERS ; BERTONI
AbstractTwo-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography (2D-RPLC/HILIC) system was successfully applied for comprehensive characterization of steviol glycosides from Stevia rebaudiana. The experiments were performed in offline mode using an XCharge C18 column in first dimension and an XAmide column in second dimension. In first dimension, preliminary separation of Stevia aqueous extract was accomplished and 30 fractions were collected. Then fractions 120 were selected for further purification and 13 compounds with high purity were obtained in second dimension. Comprehensive characterization of these compounds was completed by determination of their retention time, accurate molecular weight, diagnostic fragmentation ions, and nuclear magnetic resonance spectroscopy. As a result, all nine known steviol glycosides, as well as other four steviol glycosides were fully purified. The result demonstrated that this procedure is an effective approach for the preparative separation and comprehensive characterization of steviol glycosides in Stevia. This 2D-RPLC/HILIC method will be a promising tool for the purification of low-abundance compounds from natural products.
Language英语
WOS IDWOS:000306493700015
Citation statistics
Cited Times:44[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://cas-ir.dicp.ac.cn/handle/321008/143118
Collection中国科学院大连化学物理研究所
Affiliation1.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Nat Med Liaoning Prov, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China
Recommended Citation
GB/T 7714
Fu, Qing,Guo, Zhimou,Zhang, Xiuli,et al. Comprehensive characterization of Stevia Rebaudiana using two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2012,35(14):1821-1827.
APA Fu, Qing,Guo, Zhimou,Zhang, Xiuli,Liu, Yanfang,&Liang, Xinmiao.(2012).Comprehensive characterization of Stevia Rebaudiana using two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography.JOURNAL OF SEPARATION SCIENCE,35(14),1821-1827.
MLA Fu, Qing,et al."Comprehensive characterization of Stevia Rebaudiana using two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography".JOURNAL OF SEPARATION SCIENCE 35.14(2012):1821-1827.
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