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题名: Comprehensive characterization of Stevia Rebaudiana using two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography
作者: Fu, Qing1, 2;  Guo, Zhimou2;  Zhang, Xiuli2;  Liu, Yanfang2;  Liang, Xinmiao1, 2
关键词: Characterization ;  HILIC ;  RP ;  Stevia rebaudiana ;  Two-dimensional liquid chromatography (2D-LC)
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2012-07-01
DOI: 10.1002/jssc.201101103
卷: 35, 期:14, 页:1821-1827
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: Two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography (2D-RPLC/HILIC) system was successfully applied for comprehensive characterization of steviol glycosides from Stevia rebaudiana. The experiments were performed in offline mode using an XCharge C18 column in first dimension and an XAmide column in second dimension. In first dimension, preliminary separation of Stevia aqueous extract was accomplished and 30 fractions were collected. Then fractions 120 were selected for further purification and 13 compounds with high purity were obtained in second dimension. Comprehensive characterization of these compounds was completed by determination of their retention time, accurate molecular weight, diagnostic fragmentation ions, and nuclear magnetic resonance spectroscopy. As a result, all nine known steviol glycosides, as well as other four steviol glycosides were fully purified. The result demonstrated that this procedure is an effective approach for the preparative separation and comprehensive characterization of steviol glycosides in Stevia. This 2D-RPLC/HILIC method will be a promising tool for the purification of low-abundance compounds from natural products.
关键词[WOS]: MASS-SPECTROMETRY ;  FLUID EXTRACTION ;  PLANT-ORIGIN ;  STEVIOSIDE ;  GLYCOSIDES ;  SEPARATION ;  PEPTIDES ;  LEAVES ;  SWEETENERS ;  BERTONI
语种: 英语
WOS记录号: WOS:000306493700015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143118
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Nat Med Liaoning Prov, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China

Recommended Citation:
Fu, Qing,Guo, Zhimou,Zhang, Xiuli,et al. Comprehensive characterization of Stevia Rebaudiana using two-dimensional reversed-phase liquid chromatography/hydrophilic interaction liquid chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2012,35(14):1821-1827.
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