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题名: Oxidative Decarboxylation of L-Malate by Using a Synthetic Bioredox System
作者: Ji Debin2;  Wang Lei2;  Zhou Yongjin2;  Yang Wei;  Wang Qian1;  Zhao Zongbao1
关键词: biocatalysis ;  crude cell lysate ;  malic enzyme ;  pyruvate ;  synthetic bioredox system
刊名: CHINESE JOURNAL OF CATALYSIS
发表日期: 2012-03-01
DOI: 10.3724/SP.J.1088.2012.10938
卷: 33, 期:3, 页:530-535
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences ;  Technology
类目[WOS]: Chemistry, Applied ;  Chemistry, Physical ;  Engineering, Chemical
研究领域[WOS]: Chemistry ;  Engineering
英文摘要: A synthetic bioredox system for the oxidative decarboxylation of L-malate to pyruvate was presented, with which the enzyme purification steps can be omitted. The bioredox system consisted of ME-t (mutant malic enzyme ME L310R/Q401C) and non-natural coenzyme nicotinamide flucytosine dinucleotide (NFCD+) catalyzes the oxidation of L-malate to pyruvate and concurrently generates the reduced coenzyme, NFCDH. Escherichia coli BL21(DE3) pET24b-ME-t cell lysates catalyze the oxidation of L-malate and produce pyruvate only, indicating that the cell lysates have the same substrate selectivity as the purified enzyme. NADH oxidase (NOX) from Enterococcus faecalis can oxidize NFCDH to regenerate NFCD+. Compared with the reaction system containing NAD(+), ME lysates, and NOX lysates, the system containing NFCD+, ME-t lysates, and NOX lysates converts L-malate to pyruvate in 9% higher yield and with significantly less lactate formation. These results suggest that the synthetic bioredox system composed of ME-t and NFCD+ is easy to use and has higher product selectivity than the corresponding natural system dependent on NAD(+). The synthetic bioredox system has the potential to substitute the purified enzyme. The results provide new opportunities for design and application of redox biocatalysts.
关键词[WOS]: NADH OXIDASE ;  COFACTOR REGENERATION ;  STEREOCHEMICAL CONTROL ;  ENZYMATIC REDUCTION ;  PYRUVATE PRODUCTION ;  ENZYMES ;  PURIFICATION ;  EXPRESSION ;  YEAST
语种: 英语
WOS记录号: WOS:000302114300021
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143134
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian Natl Lab Clean Energy, Dalian 116023, Liaoning, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

Recommended Citation:
Ji Debin,Wang Lei,Zhou Yongjin,et al. Oxidative Decarboxylation of L-Malate by Using a Synthetic Bioredox System[J]. CHINESE JOURNAL OF CATALYSIS,2012,33(3):530-535.
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