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题名: Liquid-liquid/solid three-phase high-speed counter-current chromatography, a new technique for separation of polyphenols from Geranium wilfordii Maxim
作者: Liu, Dan1, 2;  Ma, Yan3;  Gu, Ming1;  Janson, Jan-Christer4;  Wang, Changhai5;  Xiao, Hongbin2
关键词: Corilagin ;  Geraniin ;  High-speed counter-current chromatography ;  Liquid-liquid ;  solid three-phase system ;  Polyphenols
刊名: JOURNAL OF SEPARATION SCIENCE
发表日期: 2012-08-01
DOI: 10.1002/jssc.201200283
卷: 35, 期:16, 页:2146-2151
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology ;  Physical Sciences
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
英文摘要: High-speed counter-current chromatography using a new liquidliquid/solid three-phase system was used for the separation of the polyphenols corilagin and geraniin from a crude extract of Geranium wilfordii Maxim in one step. The optimized three-phase system was composed of n-hexane/ethyl acetate/methanol/acetic acid/water and to which was added 10-mu m average diameter microspheres of cross-linked 12% agarose at the ratio of 0.2:10:2:1:5 and 0.1 g/mL, respectively. The purities of geraniin and corilagin were 82 and 90%, which were determined by HPLC at 280 nm. A 14.5 and 7 mg of geraniin and corilagin were purified from 160 mg crude extract with the yields of 70 and 78%, respectively.
关键词[WOS]: LINKED 12-PERCENT AGAROSE ;  ONE-STEP PURIFICATION ;  BOND ADSORPTION CHROMATOGRAPHY ;  SALVIANOLIC ACID-B ;  3,4-DIHYDROXYPHENYLLACTIC ACID ;  PHYLLANTHUS-URINARIA ;  HYDROLYZABLE TANNINS ;  GEL MEDIA ;  CORILAGIN ;  PROTOCATECHUALDEHYDE
语种: 英语
WOS记录号: WOS:000307551300020
Citation statistics: 
内容类型: 期刊论文
URI标识: http://cas-ir.dicp.ac.cn/handle/321008/143147
Appears in Collections:中国科学院大连化学物理研究所_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian, Peoples R China
3.Liaoning Prov Dalian Ocean Fishery Grp Corp, Dalian, Peoples R China
4.Uppsala Univ, Dept Phys & Analyt Chem, Uppsala, Sweden
5.Nanjing Agr Univ, Nanjing, Peoples R China

Recommended Citation:
Liu, Dan,Ma, Yan,Gu, Ming,et al. Liquid-liquid/solid three-phase high-speed counter-current chromatography, a new technique for separation of polyphenols from Geranium wilfordii Maxim[J]. JOURNAL OF SEPARATION SCIENCE,2012,35(16):2146-2151.
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